6.5.1.7: DNA ligase (ATP, ADP or GTP)

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For detailed information about DNA ligase (ATP, ADP or GTP), go to the full flat file.

Reaction

5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m

Synonyms

Hbu DNA ligase, multiple cofactor-dependent DNA ligase, Szi DNA ligase

ECTree

6 Ligases

6.5 Forming phosphoric-ester bonds

6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

6.5.1.7 DNA ligase (ATP, ADP or GTP)

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43	AA Sequence
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1	Crystallization (Commentary)
4	Metals/Ions
2	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
5	Organism
2	pH Optimum
2	pH Range
3	Purification (Commentary)
18	Reaction
5	Reference
24	Substrates and Products (Substrate)
4	Subunits
5	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
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4	Temperature Stability [°C]

Systematic Name

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Systematic Name on EC 6.5.1.7 - DNA ligase (ATP, ADP or GTP)

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poly(deoxyribonucleotide)-3'-hydroxyl:5'-phospho-poly(deoxyribonucleotide) ligase (ATP, ADP or GTP)

The enzymes from the archaea Hyperthermus butylicus and Sulfophobococcus zilligii are active with ATP, ADP or GTP. They show no activity with NAD+. The enzyme catalyses the ligation of DNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, ADP, or GTP, forming a phosphoramide bond between adenylate/guanylate and a lysine residue. The nucleotide is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine/guanosine)-[DNA]. Finally, the enzyme catalyses a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the nucleotide. Different from EC 6.5.1.1, DNA ligase (ATP), and EC 6.5.1.6, DNA ligase (ATP or NAD+), which cannot utilize GTP.