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6.5.1.7: DNA ligase (ATP, ADP or GTP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP, ADP or GTP), go to the full flat file.

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

Hbu DNA ligase, multiple cofactor-dependent DNA ligase, Szi DNA ligase

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.7 DNA ligase (ATP, ADP or GTP)

Cloned

Cloned on EC 6.5.1.7 - DNA ligase (ATP, ADP or GTP)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed under control of the T7lac promoter of pTARG in Escherichia coli BL21-Codon-Plus(DE3)-RIL
-
expression in Escherichia coli with a hexahistidine tag at the C-terminus
-