6.5.1.4: RNA 3'-terminal-phosphate cyclase (ATP)
This is an abbreviated version!
For detailed information about RNA 3'-terminal-phosphate cyclase (ATP), go to the full flat file.
Word Map on EC 6.5.1.4
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6.5.1.4
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phosphodiester
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cyclization
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ligases
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nucleotidylation
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adenylylation
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cyclases
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polynucleotide
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eucarya
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pre-rrna
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oligoribonucleotides
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ribozyme
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3',5'-phosphodiester
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alpha-32patp
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cyclase-like
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2'-phosphate
- 6.5.1.4
-
phosphodiester
-
cyclization
- ligases
-
nucleotidylation
-
adenylylation
- cyclases
- polynucleotide
- eucarya
- pre-rrna
- oligoribonucleotides
-
ribozyme
-
3',5'-phosphodiester
-
alpha-32patp
-
cyclase-like
-
2'-phosphate
Reaction
Synonyms
PH1529, Rcl1p, RNA 3'-phosphate cyclase, RNA 3'-teminal phosphate cyclase, RNA 3'-terminal phosphate cyclase, RNA 3'-terminal phosphate cylase, RNA cyclase, RNA-3'-phosphate cyclase, RNL, Rtc, Rtc1, RtcA, St-Rtc
ECTree
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Engineering
Engineering on EC 6.5.1.4 - RNA 3'-terminal-phosphate cyclase (ATP)
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F251A
considerably reduced activity compared to wild-type enzyme, about 30%
H309A
P131G
considerably reduced activity compared to wild-type enzyme, about 35%
Y284A
considerably reduced activity compared to wild-type enzyme (12%)
E14A
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activity very low, only 4% of RNA is cyclized compared to 95% with wild-type enzyme
H320A
H309A
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mutant is unreactive with either 3'-phosphate RNA end or 2'-phosphate RNA end
H320A
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mutant is unreactive with either 3'-phosphate RNA end or 2'-phosphate RNA end