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6.5.1.4: RNA 3'-terminal-phosphate cyclase (ATP)

This is an abbreviated version!
For detailed information about RNA 3'-terminal-phosphate cyclase (ATP), go to the full flat file.

Word Map on EC 6.5.1.4

Reaction

ATP
+
[RNA 3'-phosphate cyclase]-L-histidine
=
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine
+
diphosphate

Synonyms

PH1529, Rcl1p, RNA 3'-phosphate cyclase, RNA 3'-teminal phosphate cyclase, RNA 3'-terminal phosphate cyclase, RNA 3'-terminal phosphate cylase, RNA cyclase, RNA-3'-phosphate cyclase, RNL, Rtc, Rtc1, RtcA, St-Rtc

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.4 RNA 3'-terminal-phosphate cyclase (ATP)

Engineering

Engineering on EC 6.5.1.4 - RNA 3'-terminal-phosphate cyclase (ATP)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C308A
recombinant mutant protein
D287A
activity very low, 1% of wild-type enzyme
E270A
reduced activity compared to wild-type enzyme, about 80%
F135A
activity very low, 3% of wild-type enzyme
F251A
considerably reduced activity compared to wild-type enzyme, about 30%
H309A
H309G
enzyme inactive
P131G
considerably reduced activity compared to wild-type enzyme, about 35%
Q104A
almost same activiy like wild-type enzyme
Q288A
activity very low, 2% of wild-type enzyme
S129A
almost same activiy like wild-type enzyme
Y284A
considerably reduced activity compared to wild-type enzyme (12%)
D297A
-
enzyme inactive
E14A
-
activity very low, only 4% of RNA is cyclized compared to 95% with wild-type enzyme
H320A
H326A
-
almost same activiy like wild-type enzyme
H52A
-
half activity compared to wild-type enzyme
Q51A
-
almost same activiy like wild-type enzyme
R21A
-
enzyme inactive
R40A
-
enzyme inactive
R43A
-
enzyme inactive
Y294A
-
enzyme inactive