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6.5.1.4: RNA 3'-terminal-phosphate cyclase (ATP)

This is an abbreviated version!
For detailed information about RNA 3'-terminal-phosphate cyclase (ATP), go to the full flat file.

Word Map on EC 6.5.1.4

Reaction

ATP
+
[RNA 3'-phosphate cyclase]-L-histidine
=
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine
+
diphosphate

Synonyms

PH1529, Rcl1p, RNA 3'-phosphate cyclase, RNA 3'-teminal phosphate cyclase, RNA 3'-terminal phosphate cyclase, RNA 3'-terminal phosphate cylase, RNA cyclase, RNA-3'-phosphate cyclase, RNL, Rtc, Rtc1, RtcA, St-Rtc

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.4 RNA 3'-terminal-phosphate cyclase (ATP)

Crystallization

Crystallization on EC 6.5.1.4 - RNA 3'-terminal-phosphate cyclase (ATP)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion technique, 2 orthorhombic crystal forms, space group P2(1)2(1)2(1), unit-cell dimensions a : 101.8 A, b : 126.6 A and c : 128.8 A, and P2(1)2(1)2 with unit cell dimensions a : 125.8 A, b : 133.5 A, c : 51.0 A
crystal structure of the enzyme in complex with a 3'-phosphate terminated RNA and adenosine in the AMP-binding pocket
crystallized in the states St-Rtc, St-Rtc+Mn, St-Rtc+ATP, St-Rtc+AMP and St-Rtc-AMP
-
native enzyme, with AMP covalently bound, in complex with AMP, with ATP, or Mn2+, at 2.25 A, 2.25A, 2.9 A, 2.4 A and 3.2 A resolutions, respectively. Upon binding of Mg2+ to residue E10, the triphosphate group of the trapped ATP changes its conformation, with help of ligands R17 and R39. The Nepsilon atom of H307 attacks the alpha-phosphate group to form a new P-N bond. When a truncated RNA is bound, its 3'-phosphate group may be forced to react with the phosphate group of AMP, and the activiated 3'-phosphate group may be attacked by the 2'-hydroxyl group to generate the 2',3'-cyclic phosphodiester
-