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6.5.1.3: RNA ligase (ATP)

This is an abbreviated version!
For detailed information about RNA ligase (ATP), go to the full flat file.

Word Map on EC 6.5.1.3

Reaction

(ribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(ribonucleotide)m
=
(ribonucleotide)n+m
 +
AMP

Synonyms

ATP-dependent RNA ligase, b1-10t, bacteriophage RNA ligase, band IV protein, class I ligase, class I RNA ligase ribozyme, DraRnI, DraRnl, DREL, gp24.1, P52, phage Rnl2, Polynucleotide synthetase, Polyribonucleotide ligase, Polyribonucleotide synthase (ATP), REL1, Ribonucleic ligase, ribonucleprotein editing complex, RM378 RNA ligase, RNA editing ligase 1, RNA ligase, RNA ligase (ATP), RNA ligase 1, RNA ligase 2, RNA ligase ribozyme, RNA-editing ligase 1, RNL, Rnl1, Rnl2, Rnl5, RnlA, RtcA, rtcB, Synthetase, polyribonucleotide, T4 RNA ligase, T4 RNA ligase 1, T4 RNA ligase 2, T4Rnl2, TbMP52, TbREL1, thermostable RNA ligase 1, Trl1

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.3 RNA ligase (ATP)

Cloned

Cloned on EC 6.5.1.3 - RNA ligase (ATP)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
encoded by T4 gene 24.1, mutants expressed in Escherichia coli
Tequatrovirus T4
-
enzymatically active Leishmania tarentolae REL1 and REL2 and Trypanosoma brucei REL1 proteins from a Baculovirus expression system. The recombinant proteins can each integrate into an L-complex that has been depleted of the cognate ligase by RNAi. In the case of REL1, the integration of the recombinant enzyme functionally complements the REL1-depleted L-complex in an in vitro editing system
expressed in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli as a His10-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Sf9 insect cells
expressed in Trypanosoma brucei lines as an epitope-tagged fusion protein, recombinant p48 also expressed in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli BL21goldDE3
-
overexpressed in Escherichia coli BL21 (DE3)
Tequatrovirus T4
-
RNA ligase gene is inserted into a pET23b expression vector with a C-terminal His6-tag. This vector is transformed into Escherichia coli strain BL21
-
RnlA gene overexpressed in Escherichia coli BL21
-
transformed into Escherichia coli BL21 (DE3)
Tequatrovirus T4
-
transformed into Escherichia coli BL21(DE3)
Tequatrovirus T4
-