Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Temperature Optimum

Temperature Optimum on EC 6.5.1.2 - DNA ligase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 15
-
ligation of cohesive ends
20
-
mutant enzyme L15F has 20fold lower ligation activity in vitro than wild-type enzyme
24 - 37
-
cohesive-end DNA restriction fragments
27.7
-
p(dT)8, joining of thymidylate oligomers in the presence of poly(dA) as a template
34.5
-
p(dT)10, joining of thymidylate oligomers in the presence of poly(dA) as a template
42
-
mutant enzyme L15F has 60fold lower ligation activity in vitro than wild-type enzyme
65 - 72
-
nick-closing activity
70
-
mutant enzyme DELTA582-667 and wild-type enzyme, activity of mutant enzyme DELTA582-667 is 50% of wild-type activity