6.5.1.2: DNA ligase (NAD+)

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For detailed information about DNA ligase (NAD+), go to the full flat file.

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Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases

         6.5 Forming phosphoric-ester bonds

             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

                6.5.1.2 DNA ligase (NAD+)

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Results	in table
16	Activating Compound
48846	AA Sequence
22	Application
1	CAS Registry Number
51	Cloned(Commentary)
120	Cofactor
13	Crystallization (Commentary)
1	Expression
3	General Information
1	General Stability
449	IC50 Value
614	Inhibitors
7	Ki Value [mM]
38	KM Value [mM]
85	Metals/Ions
27	Molecular Weight [Da]
112	Natural Substrates/ Products (Substrates)
877	Organism
56	PDB ID
20	pH Optimum
8	pH Range
1	pH Stability
183	Protein Variants
51	Purification (Commentary)
32	Reaction
1	Reaction Type
193	Reference
5	Specific Activity [micromol/min/mg]
5	Storage Stability
245	Substrates and Products (Substrate)
13	Subunits
249	Synonyms
1	Systematic Name
24	Temperature Optimum [°C]
10	Temperature Range [°C]
16	Temperature Stability [°C]
15	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 6.5.1.2 - DNA ligase (NAD+)

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SYSTEMATIC NAME

IUBMB Comments

poly(deoxyribonucleotide)-3'-hydroxyl:5'-phospho-poly(deoxyribonucleotide) ligase (NAD+)

The enzyme, typically found in bacteria, catalyses the ligation of DNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by NAD+, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[DNA]. Finally, the enzyme catalyses a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate. RNA can also act as substrate, to some extent. cf. EC 6.5.1.1, DNA ligase (ATP), EC 6.5.1.6, DNA ligase (ATP or NAD+), and EC 6.5.1.7, DNA ligase (ATP, ADP or GTP).