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6.5.1.2: DNA ligase (NAD+)

This is an abbreviated version!
For detailed information about DNA ligase (NAD+), go to the full flat file.

Word Map on EC 6.5.1.2

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
+
AMP

Synonyms

beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.2 DNA ligase (NAD+)

Cloned

Cloned on EC 6.5.1.2 - DNA ligase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1-318 fragment with K114A mutation
-
adenylation domain
capable of complementing DNA-ligase-deficient yeast, wild type and mutants expressed in Escherichia coli BL21(DE3)
-
deletion mutants, expression in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3)
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL26
-
expressed in Escherichia coli GR501, that contains a temperature sensitive mutation in ligA and is therefore unable to grow above 42°C, expression of protein enables Escherichia coli GR501 at 44°C
expressed in Escherichia coli GR501, that contains a temperature sensitive mutation in ligA and is therefore unable to grow above 42°C, expression of protein enables Escherichia coli GR501 to grow at 44°C
-
expressed in Escherichia coli Rosetta cells
Wolbachia sp. subsp. Brugia malayi
expression in Escherichia coli
full length and truncated enzymes expressed in Escherichia coli BL21 (DE3)
-
ligase fragments and mutant
-
N-terminal fragment 1-318 and C-terminal fragment starting from position 397 seperately expressed in Escherichia coli BL21
-
native and mutant enzymes in Escherichia coli BL21 (DE3)
-
native and selenomethionine-substituted enzyme
overexpressed as a His-tagged fusion protein in Escherichia coli
-
overexpression in Escherichia coli
produced in Escherichia coli as a His10-LigA fusion
-
recombinantly expressed
wild type and fragments expressed in Escherichia coli GI724
-
wild-type and mutant forms of LigN are expressed in Escherichia coli
-
wild-type and mutant proteins Y36A, D46A, Y49A, D50A, and K113A are produced in Escherichia coli
-