6.5.1.2: DNA ligase (NAD+) This is an abbreviated version! For detailed information about DNA ligase (NAD+), go to the full flat file .
Reaction
(deoxyribonucleotide)n-3'-hydroxyl +
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m =
(deoxyribonucleotide)n+m +
AMP
Synonyms beta-NAD+ -dependent DNA ligase, beta-NAD+-dependent DNA ligase, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase (NAD), DNA repair enzyme, DNA-joining enzyme, LigA, Ligase, polynucleotide (nicotinamide adenine dinucleotide), LigN, MimiLIG, MsEPV DNA ligase, MtuLigA, NAD(+)-dependent DNA ligase, NAD+ dependent DNA ligase, NAD+ DNA ligase, NAD+-dependent DNA ligase, NAD+-dependent DNA ligase A, NAD-dependent DNA ligase, NDL, Polydeoxyribonucleotide synthase (NAD+), Polydeoxyribonucleotide synthase [NAD+], Polynucleotide ligase, Polynucleotide synthetase, Polynucleotide synthetase (nicotinamide adenine dinucleotide), Rv3014c, Synthetase, polydeoxyribonucleotide (nicotinamide adenine dinucleotide), T4 DNA ligase, Taq DNA ligase, Tfi DNA ligase, Tth DNA ligase, wBm-LigA
ECTree
Application
Application on EC 6.5.1.2 - DNA ligase (NAD+)
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biotechnology
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LigN is unique amongst DNA ligase enzymes in displaying maximal DNA strand joining activity at above 3 M salt levels. As such the LigN enzyme has potential both as a novel tool for biotechnology and as a model enzyme for studying the adaptation of proteins to high intracellular salt levels
analysis
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DNA ligase is an essential reagent in studies on nucleic acid structure and metabolism
analysis
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DNA ligase, in combination with polynucleotide kinase, can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis
analysis
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DNA ligase can be used to determine the ability of other enzymes to act at nicks and gaps in duplex DNA molecules
analysis
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DNA ligase can be used to study the primary and secondary structure of DNA molecules
analysis
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high-throughput assay for the adenylation reaction of bacterial DNA ligase
medicine
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potential target for antibiotics
medicine
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potential target for antibiotics
medicine
potential target for antibiotics
medicine
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potential target for antibiotics due to the fact that eukaryotic cells use ATP instead of NAD+ as cofactor for DNA-ligase
medicine
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potential target for antiobiotics
medicine
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although NAD+-dependent DNA ligase is essential for mycobacterial viability, only low levels of protein are required for growth. very efficient inhibition of enzyme activity would be required if NAD+-dependent DNA ligase is to be useful as an antibiotic target in mycobacteria
medicine
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NAD+-dependent ligases can serve as a valuable target in the development if chemotherapeutics for the treatment of numerous human ailments
medicine
NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments
medicine
NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments
medicine
NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments
medicine
NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments
medicine
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NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments
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synthesis
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DNA ligase is an indispensible reagent in the chemical synthesis of double-stranded DNA of specific nucleotide sequence
synthesis
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an important use of DNA ligase is the preparation of recombinant DNA molecules for use in the cloning of DNA
synthesis
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DNA ligase is used for cDNA cloning by replacement synthesis