6.5.1.1: DNA ligase (ATP)

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For detailed information about DNA ligase (ATP), go to the full flat file.

Reaction

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases

         6.5 Forming phosphoric-ester bonds

             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

                6.5.1.1 DNA ligase (ATP)

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Results	in table
34	Activating Compound
47549	AA Sequence
9	Application
1	CAS Registry Number
86	Cloned(Commentary)
120	Cofactor
23	Crystallization (Commentary)
1953	Disease/ Diagnostics
2	Expression
34	General Information
9	General Stability
19	IC50 Value
177	Inhibitors
8	kcat/KM [mM/s]
1	Ki Value [mM]
77	KM Value [mM]
39	Localization
119	Metals/Ions
71	Molecular Weight [Da]
164	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
348	Organism
66	PDB ID
37	pH Optimum
11	pH Range
1	pH Stability
2	pI Value
1	Posttranslational Modification
141	Protein Variants
105	Purification (Commentary)
9	Reaction
1	Reaction Type
340	Reference
111	Source Tissue
8	Specific Activity [micromol/min/mg]
8	Storage Stability
445	Substrates and Products (Substrate)
72	Subunits
395	Synonyms
1	Systematic Name
30	Temperature Optimum [°C]
10	Temperature Range [°C]
25	Temperature Stability [°C]
30	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 6.5.1.1 - DNA ligase (ATP)

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

100

2 entries

37

2 entries

38

Tequatrovirus T4

-

inactivation temperature, Q10: 2.3

1919

4

Chlorocebus aethiops

-

14 days, autoproteolysis is observed

1911

40

Bos taurus

-

DNA ligase II, rapid loss of activity above

1927

42

3 entries

50

Bos taurus

-

DNA ligase I is stable to prolonged heating

1927

52

Bos taurus

-

DNA ligase I, 5 min, 50% inactivation

1927

80

3 entries

85

2 entries

90

3 entries

94

2 entries

95

2 entries

99

Hyperthermus butylicus

-

Hbu DNA ligase exhibtis a 1.7 h half life at 99 °C

727438

100

Aeropyrum pernix

Q9YD18

stable for 60 min, half-life: 25 min

727504

100

Deinococcus radiodurans

-

the half-life of heat inactivation at 100°C is about 5 min

694991

37

Chlorocebus aethiops

-

24 h, autoproteolysis is observed

1911

37

Vaccinia virus

-

significant loss of ligation activity

650937

42

Bos taurus

-

-

1906

42

Bos taurus

-

DNA ligase II, 5 min, 50% inactivation

1927

42

Mammalia

-

rapid inactivation, DNA ligase II

1906

80

Saccharolobus shibatae

Q9P9K9

15% loss of activity after 5 h

651216

80

Thermococcus sp.

C0LJI8

more than 50% Lig1519 activity is preserved after incubation of the enzyme at 80°C for 30 min

690511

80

uncultured archaeon

-

5 h, about 5% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene

726420

85

Saccharolobus shibatae

Q9P9K9

29% loss of activity after 5 h

651216

85

uncultured archaeon

-

5 h, about 10% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene

726420

90

Methanocaldococcus jannaschii

Q57635

20 min, about 20% loss of activity

728739

90

Saccharolobus shibatae

Q9P9K9

50% loss of activity after approx. 10 min

651216

90

uncultured archaeon

-

1 h, about 55% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene

726420

94

Hyperthermus butylicus

-

Hbu DNA ligase exhibits about 50% of the original activity for 2.3 h at temperatures up to 94 °C

727438

94

Thermococcus sp. 1519

C0LJI8

the unmodified enzyme loses half of its activity upon 8 min incubation at 94°C

747085

95

Pyrococcus abyssi

P0CL74

15 min, inactivated

731171

95

uncultured archaeon

-

10 min, about 80% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene

726420