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6.5.1.1: DNA ligase (ATP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP), go to the full flat file.

Word Map on EC 6.5.1.1

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.1 DNA ligase (ATP)

KM Value

KM Value on EC 6.5.1.1 - DNA ligase (ATP)

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
(2E,6E)-farnesyl triphosphate
Tequatrovirus T4
-
-
0.000029
(deoxyribonucleotide)20
-
25°C, pH 7.5
-
0.00009
(dT)20
-
hybridized with (dA)
0.000124
(nicked double-stranded DNA)n
at 30°C, pH not specified in the publication
-
0.000001
(single-stranded DNA splinted by RNA)m
-
value below, pH 7.4, 25°C
-
0.0000034 - 1
ATP
0.08
clodronate
Tequatrovirus T4
-
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C
0.0016 - 0.004
dATP
4
dimethylallyl diphosphate
Tequatrovirus T4
-
-
0.0000015 - 0.05
DNA
0.73
etidronate
Tequatrovirus T4
-
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C
0.05
farnesyl diphosphate
Tequatrovirus T4
-
-
0.21
geranyl diphosphate
Tequatrovirus T4
-
-
0.31
geranyl triphosphate
Tequatrovirus T4
-
-
0.4
GTP
pH 7.5, 70°C, synthesis of P1-(5'-adenosyl),P4-(5'-guanosyl)tetraphosphate
2.3
isopentenyl diphosphate
Tequatrovirus T4
-
-
0.63
isopentenyl triphosphate
Tequatrovirus T4
-
-
0.024
methylenebisphosphonate
Tequatrovirus T4
-
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C
0.0000026 - 0.0014
nicked DNA
-
1.3
tripolyphosphate
Tequatrovirus T4
-
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C
additional information
additional information
-