6.5.1.1: DNA ligase (ATP)

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For detailed information about DNA ligase (ATP), go to the full flat file.

Reaction

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases

         6.5 Forming phosphoric-ester bonds

             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)

                6.5.1.1 DNA ligase (ATP)

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Results	in table
34	Activating Compound
47549	AA Sequence
9	Application
1	CAS Registry Number
86	Cloned(Commentary)
120	Cofactor
23	Crystallization (Commentary)
1953	Disease/ Diagnostics
2	Expression
34	General Information
9	General Stability
19	IC50 Value
177	Inhibitors
8	kcat/KM [mM/s]
1	Ki Value [mM]
77	KM Value [mM]
39	Localization
119	Metals/Ions
71	Molecular Weight [Da]
164	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
348	Organism
66	PDB ID
37	pH Optimum
11	pH Range
1	pH Stability
2	pI Value
1	Posttranslational Modification
141	Protein Variants
105	Purification (Commentary)
9	Reaction
1	Reaction Type
340	Reference
111	Source Tissue
8	Specific Activity [micromol/min/mg]
8	Storage Stability
445	Substrates and Products (Substrate)
72	Subunits
395	Synonyms
1	Systematic Name
30	Temperature Optimum [°C]
10	Temperature Range [°C]
25	Temperature Stability [°C]
30	Turnover Number [1/s]

General Stability

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General Stability on EC 6.5.1.1 - DNA ligase (ATP)

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

a 10 U/ml dilution loses 40% of its activity in 3 months

Tequatrovirus T4

-

1910

complexes of the enzyme variants with either double- and single-stranded DNA fragments are less stable than the native DNA ligase

Pyrococcus abyssi

P0CL74

731171

DNA ligase I is highly sensitive to partial proteolysis

Mammalia

-

1906

DNA ligase IV depends on the partner protein XRCC4 for stability and activity

Homo sapiens

P18858

673070

Lig3 stability depends upon its nuclear binding partner Xrcc1

Mus musculus

-

716334

retains 90% of its activity after 3 cycles of freeze-thawing

Drosophila melanogaster

-

1914

T4 DNA ligase gradually lose their activity when incubated at temperatures above 0°C

Tequatrovirus T4

-

651161

Tween 20, 0.2% or bovine serum albumin stabilizes

Mammalia

-

1906

very susceptible to proteolysis

Drosophila melanogaster

-

1906