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6.4.1.6: acetone carboxylase

This is an abbreviated version!
For detailed information about acetone carboxylase, go to the full flat file.

Word Map on EC 6.4.1.6

Reaction

acetone
+
CO2
+
ATP
+ 2 H2O =
acetoacetate
+
AMP
+ 2 phosphate

Synonyms

acetone carboxylase, acetone-carboxylating enzyme, ACX, AcxA, AcxB, AcxC, Alide_1502, Alide_1503, Alide_1504, carboxylase, acetone

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.6 acetone carboxylase

Purification

Purification on EC 6.4.1.6 - acetone carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose chromatography, but hydrophobic interaction, gel filtration and anion-exchange chromatography by Q-Sepharose resulted in large loss of activity
-
DEAE-Sepharose chromatography, gel filtration with Sephacryl S-300 column, HiLoad Q-Sepharose column
-
enriched from cell extracts of Alicycliphilus denitrificans by two subsequent DEAE Sepharose column procedures
-
ion-exchange chromatography
-
using anion DEAE-Sepharose chromatography followed by Sephacryl S300 molecular filtration
-