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6.4.1.3: propionyl-CoA carboxylase

This is an abbreviated version!
For detailed information about propionyl-CoA carboxylase, go to the full flat file.

Word Map on EC 6.4.1.3

Reaction

ATP
+
propanoyl-CoA
+
HCO3-
+
H+
=
ADP
+
phosphate
+
(S)-methylmalonyl-CoA

Synonyms

AccA3-PccB complex, acetyl-CoA/propionyl-CoA carboxylase, Carboxylase, propional coenzyme A (adenosine triphosphate-hydrolyzing), LA_2736-LA_2735, Pcase, PCC, PccA, PccA-1, PCCase, PccB, PccB-1, pccBC, PccE, Propanoyl-CoA:carbon dioxide ligase, Propionyl coenzyme A carboxylase, Propionyl coenzyme A carboxylase (adenosine triphosphate-hydrolyzing), Propionyl coenzyme A carboxylase (ATP-hydrolyzing), Propionyl-CoA carboxylase, propionyl-coenzyme A carboxylase

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.3 propionyl-CoA carboxylase

Cloned

Cloned on EC 6.4.1.3 - propionyl-CoA carboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
correction of enzyme deficiency in pccA fibroblasts
-
expressed in COS-7 cells at 27°C and 37°C
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli as a His-tagged fusion protein. Recombinant PCC, including the His tag are produced and are confirmed to react with clonorchiasis patient sera
-
expressed in Escherichia coli BL21 lambda(DE3) cells
expressed in Saccharomyces cerevisiae
-
expressed using a two hybrid system in COS cells
-
expression in Escherichia coli
expression of alpha and beta subunit in Escherichia coli
-
gene encoding the biotin-containing subunit
-
into plasmid pSET152 derivative containing an ermE* promoter, yielding pYJ269, expressed in Streptomyces clavuligerus CKD1119
-
introduced into Streptomyces hygroscopicus UV2-2 strain by conjugation from Escherichia coli ET12567/pUZ8002
-
subunits individually cloned and expressed in Escherichia coli DH5alpha
-
the PccB subunit is expressed in Escherichia coli BL21(DE3) cells
-