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6.4.1.1: pyruvate carboxylase

This is an abbreviated version!
For detailed information about pyruvate carboxylase, go to the full flat file.

Word Map on EC 6.4.1.1

Reaction

ATP
+
pyruvate
 +
HCO3-
 +
H+
=
ADP
 +
phosphate
 +
oxaloacetate

Synonyms

Carboxylase, pyruvate, EhPYC1, HpPyc1p, Mfla_1512, MSmeg_2412, PC, Pcase, PCB, PCC, PCx, PYC, Pyc1, Pyc1p, PYC2, pycA, pyruvate carboxylase, pyruvate carboxylase 1, Pyruvic carboxylase, RePC

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.1 pyruvate carboxylase

Temperature Stability

Temperature Stability on EC 6.4.1.1 - pyruvate carboxylase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
10 mM potassium phosphate, 10 mM KCl, 1 mM EDTA, 0.1 mM DTT, pH 6.8, potassium phosphate-KCl buffer, 50% loss of activity after 10 min
20
-
1 h, 75% inactivation
46
-
10 min, 50% loss of activity
50
-
15 min, complete loss of activity
55
-
half-life: 9 min. A mixture of Tris-HCl, potassium bicarbonate, sodium pyruvate, ATP, MgC2l, and acetyl-CoA at concentrations similar to those used in the enzyme assay, protect completely for at least 30 min
60
-
pH 7, half-life: 1 h
80
-
rapid inactivation, t1/2: 1 h in 100 mM Tris/HCl, pH 7, sulfate salts provide partial protection
additional information