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6.4.1.1: pyruvate carboxylase

This is an abbreviated version!
For detailed information about pyruvate carboxylase, go to the full flat file.

Word Map on EC 6.4.1.1

Reaction

ATP
+
pyruvate
+
HCO3-
+
H+
=
ADP
+
phosphate
+
oxaloacetate

Synonyms

Carboxylase, pyruvate, EhPYC1, HpPyc1p, Mfla_1512, MSmeg_2412, PC, Pcase, PCB, PCC, PCx, PYC, Pyc1, Pyc1p, PYC2, pycA, pyruvate carboxylase, pyruvate carboxylase 1, Pyruvic carboxylase, RePC

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.1 pyruvate carboxylase

Specific Activity

Specific Activity on EC 6.4.1.1 - pyruvate carboxylase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
-
cells grown on a phosphate-deficient medium TRP, a coupled assay with malate dehydrogenase
0.004
-
free biotin carboxylation activity of the mutant K1112A in absence of acetyl-CoA at 25 mM
0.005
0.008
-
cells grown on a phosphate-deficient medium TRP, a coupled assay with malate dehydrogenase
0.03
-
cells grown on a phosphate-sufficient minimal medium M9, a coupled assay with malate dehydrogenase
0.05
-
cell extract, expressed in NZN111 after growth under CO2-atmosphere without IPTG induction
0.056
-
cell extract, expressed in AFP111 after growth under CO2-atmosphere without IPTG induction
0.068
-
cell extract, expressed in NZN111 after growth under CO2-atmosphere and induction with IPTG
0.12
-
cell extract, expressed in AFP111 after growth under CO2-atmosphere and induction with IPTG
0.49
-
cell extract, expressed in NZN111 after aerobic growth and induction with IPTG
0.587
-
control CHO-K1 cells
0.72
-
cell extract, expressed in AFP111 after aerobic growth and induction with IPTG
0.81
-
cell extract, expressed in AFP111 after aerobic growth without IPTG induction
19.43
-
enzyme recombinantly expressed in Pseudomonas aeruginosa
43.8
-
nondiabetic subjects
47.8
-
type 2 diabetic patients
additional information