6.4.1.1: pyruvate carboxylase

This is an abbreviated version!
For detailed information about pyruvate carboxylase, go to the full flat file.

Word Map on EC 6.4.1.1

6.4.1.1

phosphoenolpyruvate

gluconeogenesis

carboxykinase

biotin

oxaloacetate

acetyl-coa

tricarboxylic

gluconeogenic

anaplerotic

malate

citrate

tca

co2

malic

pepck

carboxylases

astrocyte

glucose-6-phosphatase

biotin-dependent

acidosis

propionyl-coa

citric

krebs

glutamicum

bark

pine

biotin-containing

13c-labeled

propionyl

isotopomer

maritime

1-13cglucose

avidin

hyperammonemia

fructose-1,6-diphosphatase

carboxyltransferase

pyrogenic

4.1.1.32

1,6-bisphosphatase

holocarboxylase

transcarboxylase

biotinidase

pyrolytic

leigh

ureagenesis

medicine

glucogenic

biotechnology

penicillinase

13c-enriched

pinaster

synthesis

3-methylcrotonyl-coa

Reaction

Synonyms

Carboxylase, pyruvate, EhPYC1, HpPyc1p, Mfla_1512, MSmeg_2412, PC, Pcase, PCB, PCC, PCx, PYC, Pyc1, Pyc1p, PYC2, pycA, pyruvate carboxylase, pyruvate carboxylase 1, Pyruvic carboxylase, RePC

ECTree

6 Ligases

6.4 Forming carbon-carbon bonds

6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)

6.4.1.1 pyruvate carboxylase

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Results	in table
84	Activating Compound
17340	AA Sequence
14	Application
1	CAS Registry Number
63	Cloned(Commentary)
82	Cofactor
13	Crystallization (Commentary)
1260	Disease/ Diagnostics
1	Expression
11	General Information
13	General Stability
1	IC50 Value
119	Inhibitors
23	kcat/KM [mM/s]
14	Ki Value [mM]
127	KM Value [mM]
32	Localization
86	Metals/Ions
67	Molecular Weight [Da]
81	Natural Substrates/ Products (Substrates)
246	Organism
13	Pathway
83	PDB ID
22	pH Optimum
3	pH Range
1	Posttranslational Modification
111	Protein Variants
43	Purification (Commentary)
25	Reaction
55	Reaction Type
196	Reference
112	Source Tissue
37	Specific Activity [micromol/min/mg]
9	Storage Stability
231	Substrates and Products (Substrate)
80	Subunits
57	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Range [°C]
13	Temperature Stability [°C]
67	Turnover Number [1/s]

Purification

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Purification on EC 6.4.1.1 - pyruvate carboxylase

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21 entries

Co2+-affinity column chromatography

Rhizobium etli

713960

HisPur cobalt resin column chromatography

Rhizobium etli

714190

partial

3 entries

Pyc1 isoform and C249A mutant

Saccharomyces cerevisiae

650234

Pyc1 isoform, 88% purity

Saccharomyces cerevisiae

650151

rapid dye-ligand affinity chromatography procedure

Rhodobacter capsulatus

1791

rapid purification methods

Rattus norvegicus

1783

recombinant C-terminal region from Escherichia coli strain BL21(DE3)

Homo sapiens

P11498

694325

recombinant enzyme and enzyme from liver

Homo sapiens

P11498

649546

recombinant His9-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by affinity chromatography

Geobacillus thermodenitrificans

692637

using Ni-NTA chromatography

3 entries