6.3.5.6: asparaginyl-tRNA synthase (glutamine-hydrolysing)
This is an abbreviated version!
For detailed information about asparaginyl-tRNA synthase (glutamine-hydrolysing), go to the full flat file.
Word Map on EC 6.3.5.6
-
6.3.5.6
-
gln-trnagln
-
transamidation
-
asn-trnaasn
-
aminoacyl-trnas
-
asparagine
-
mischarged
-
glutaminyl-trna
-
aminoacylation
-
archaea
-
heterotrimeric
-
synthetases
-
nondiscriminating
-
misacylated
-
helicobacter
-
gatde
-
transamidosome
-
nd-asprs
-
glutamyl-trnagln
-
pylori
-
glutaminase
-
thermautotrophicus
-
transamidase
-
asparaginylation
-
gln-trna
-
trnaglu
-
deinococcus
-
radiodurans
-
glutaminylated
-
methanothermobacter
-
mistranslation
-
trnaasp
-
anticodon-binding
-
d-loop
-
pharmacology
-
biotechnology
- 6.3.5.6
- gln-trnagln
-
transamidation
- asn-trnaasn
- aminoacyl-trnas
- asparagine
-
mischarged
- glutaminyl-trna
- aminoacylation
- archaea
-
heterotrimeric
- synthetases
-
nondiscriminating
-
misacylated
-
helicobacter
- gatde
-
transamidosome
- nd-asprs
- glutamyl-trnagln
- pylori
- glutaminase
- thermautotrophicus
-
transamidase
-
asparaginylation
-
gln-trna
- trnaglu
- deinococcus
- radiodurans
-
glutaminylated
-
methanothermobacter
-
mistranslation
- trnaasp
-
anticodon-binding
-
d-loop
- pharmacology
- biotechnology
Reaction
Synonyms
AdT, AsnRS, Asp-AdT, Asp-tRNAAsn amidotransferase, Asp/Glu-Adt, asparaginyl-transfer RNA synthetase, Asparaginyl-tRNA synthetase, aspartyl-tRNAAsn amidotransferase, GatCAB, glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, NRS, tRNA-dependent amidotransferase
ECTree
6.3.5.6 asparaginyl-tRNA synthase (glutamine-hydrolysing)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 6.3.5.6 - asparaginyl-tRNA synthase (glutamine-hydrolysing)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + Asp-tRNAAsn + L-glutamine + H2O
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
-
?
ATP + Glu-tRNAGln + L-glutamine
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
-
-
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
tRNA-dependent transamidation pathway of Asn-tRNA formation, which is required for protein synthesis or under certain metabolic situations for asparagine synthesis
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
AdT is related to the biosynthesis of asparagine rather than to provide charged tRNA for protein biosynthesis
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
gatCAB genes encoded Asp-AdT may be responsible for biosynthesis of asparagine, glutamine as amide source
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
in vivo only Asn-tRNAAsn formation occurs
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
in vivo only Asn-tRNAAsn formation occurs
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
in vivo only Asn-tRNAAsn formation, because the substrate availability of Asp-tRNAAsn allows only one final product
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
in vivo only Asn-tRNAAsn formation occurs, since Thermus possesses a mischarging Asp-tRNA synthetase, but lacks a mischarging Glu-tRNA synthetase, and cellular activity of AdTs only depends upon presence or lack of the mischarging amino acyl-tRNA synthetases, explaining why those enzymes have not restricted their specificity
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
AdT is related to the biosynthesis of asparagine rather than to provide charged tRNA for protein biosynthesis
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
in vivo only Asn-tRNAAsn formation occurs, since Thermus possesses a mischarging Asp-tRNA synthetase, but lacks a mischarging Glu-tRNA synthetase, and cellular activity of AdTs only depends upon presence or lack of the mischarging amino acyl-tRNA synthetases, explaining why those enzymes have not restricted their specificity
-
?
Asp-tRNAAsn + ?
Asn-tRNAAsn + ?
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase
-
?
ATP + Asp-tRNA + L-Gln
?
-
in presence of tRNA-dependent amidotransferase AdT, amidation activity
-
-
?
ATP + Asp-tRNA + L-Gln
?
-
in presence of tRNA-dependent amidotransferase AdT, amidation activity
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual-specific Asp/Glu-AdT, rates for conversion of Glu to Gln are about twice as fast as the rate of Asp to Asn conversion, enzyme uses glutamine, asparagine or ammonia as amide donors in the presence of ATP, GTP or CTP
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
no restricted substrate specificity of tRNA-dependent AdT: dual-specific Asp/Glu-AdT, enzyme catalyzes transamidation of Asp-tRNAAsn, since Thermus possesses a mischarging Asp-tRNA synthetase, but lacks a mischarging Glu-tRNA synthetase, GatA subunit can discriminate between Asp-tRNAAsn and Glu-tRNAGln and therefore is implicated in binding of the tRNA
forms Asn-tRNAAsn
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Asp-tRNAAsn + glutamine
ADP + phosphate + Asn-tRNAAsn + glutamate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
no restricted substrate specificity of tRNA-dependent AdT: dual-specific Asp/Glu-AdT, enzyme catalyzes transamidation of Asp-tRNAAsn, since Thermus possesses a mischarging Asp-tRNA synthetase, but lacks a mischarging Glu-tRNA synthetase, GatA subunit can discriminate between Asp-tRNAAsn and Glu-tRNAGln and therefore is implicated in binding of the tRNA
forms Asn-tRNAAsn
?
ATP + Asp-tRNAAsn + L-glutamine
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
-
-
?
ATP + Asp-tRNAAsn + L-glutamine
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
identity elements used by GatCAB to discriminate tRNAAsn from tRNAAsp. GatCAB specifically binds Asp-tRNAAsn. Therefore, modified nucleotides do not play an essential role in GatCAB discrimination of Asp-tRNAAsn from Asp-tRNAAsp
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Gln-tRNAGln formation
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Gln-tRNAGln formation
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual-specific Asp/Glu-AdT, rates for conversion of Glu to Gln are about twice as fast as the rate of Asp to Asn conversion, enzyme uses glutamine, asparagine or ammonia as amide donors in the presence of ATP or GTP
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Asn-tRNAAsn formation
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
-
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
the enzyme has no Glu-tRNAGln substrate in its host, but it can transamidate heterologous Glu-tRNAGln
-
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
-
in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Asn-tRNAAsn formation
-
?
ATP + Glu-tRNAGln + glutamine
ADP + Gln-tRNAGln + glutamate + phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Asn-tRNAAsn formation
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
-
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
recombinantly produced tRNA substrate
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
-
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
recombinantly produced tRNA substrate
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
-
-
-
?
ATP + L-aspartyl-tRNAAsn + L-glutamine
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
the Halobacter pylori Asp/Glu-Adt can utilize Escherichia coli Asp-tRNAAsn as a substrate, recombinantly produced tRNA substrate
-
-
?
Glu-tRNAGln + ?
Gln-tRNAGln + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
Glu-tRNAGln + ?
Gln-tRNAGln + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
Glu-tRNAGln + ?
Gln-tRNAGln + ?
-
tRNA-dependent transamidation pathway of Gln-tRNA formation, which is required for protein synthesis or under certain metabolic situations for glutamine synthesis
-
?
Glu-tRNAGln + ?
Gln-tRNAGln + ?
-
it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo
-
?
additional information
?
-
-
archaeal enzyme resembles the bacterial Glu/Asp-AdT, but can only amidate Asp-tRNAAsn and not Glu-tRNAGln
-
-
?
additional information
?
-
-
archaeal enzyme resembles the bacterial Glu/Asp-AdT, but can only amidate Asp-tRNAAsn and not Glu-tRNAGln
-
-
?
additional information
?
-
-
the parasite Brugia malayi enzyme, in analogy to the human enzyme, induces human leukocyte chemotaxis and activates G-protein-coupled receptors CXCR1 and CXCR2, but not CXCR3, filarial asparaginyl-tRNA synthetase, AsnRS, is known to be an immunodominant antigen that induces strong human immunoglobulin G3 responses and contributes to the development of chronic inflammatory disease such as lymphatic filariasis, overview
-
-
?
additional information
?
-
-
GatA is likely to be the catalytic subunit, GatB may be responsible for tRNA binding and GatC may be involved in a channeling mechanism, in which the misacylated tRNA formed by the non-discriminating AA-tRNA synthetase could be handed off to the AdT
-
-
?
additional information
?
-
-
no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu
-
-
?
additional information
?
-
-
no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu
-
-
?
additional information
?
-
-
enzyme specificity depends on the biochemical context, i.e. on the availability of the mischarged aminoacyl-tRNA substrate, which is controlled by the presence of a nondiscriminating GluRS or AspRS
-
-
?
additional information
?
-
-
no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu
-
-
?
additional information
?
-
-
A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein
-
-
?
additional information
?
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein
-
-
?
additional information
?
-
-
in bacteria that lack AsnRS, EC 6.1.1.12, AspRS is nondiscriminating, EC 6.1.1.23, and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, overview
-
-
?
additional information
?
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in bacteria that lack AsnRS, EC 6.1.1.12, AspRS is nondiscriminating, EC 6.1.1.23, and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, overview
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additional information
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in bacteria that lack AsnRS, EC 6.1.1.12, AspRS is nondiscriminating, EC 6.1.1.23, and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, overview
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additional information
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human cytoplasmic aminoacyl-tRNA synthetases, which are autoantigens in idiopathic inflammatory myopathies, activate chemokine receptors on T lymphocytes, monocytes, and immature dendritic cells by recruiting immune cells that could induce innate and adaptive immune responses
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archaeal enzyme resembles the bacterial Glu/Asp-AdT, but can only amidate Asp-tRNAAsn and not Glu-tRNAGln
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archaeal enzyme resembles the bacterial Glu/Asp-AdT, but can only amidate Asp-tRNAAsn and not Glu-tRNAGln
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no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu
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no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu
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a nondiscriminating aspartyl-tRNA synthetase, ND-DRS, first generates a mischarged aspartyl-tRNAAsn that dissociates from the enzyme and binds to a tRNA-dependent amidotransferase, AdT, which then converts the tRNA-bound aspartate into asparagine, the ND-DRS, tRNAAsn, and AdT assemble into a specific ribonucleoprotein complex called transamidosome that remains stable during the overall catalytic process, overview
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enzyme specificity depends on the biochemical context, i.e. on the availability of the mischarged aminoacyl-tRNA substrate, which is controlled by the presence of a nondiscriminating GluRS or AspRS
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cellular activity of AdTs only depends upon presence or lack of the mischarging amino acyl-tRNA synthetases
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additional information
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a nondiscriminating aspartyl-tRNA synthetase, ND-DRS, first generates a mischarged aspartyl-tRNAAsn that dissociates from the enzyme and binds to a tRNA-dependent amidotransferase, AdT, which then converts the tRNA-bound aspartate into asparagine, the ND-DRS, tRNAAsn, and AdT assemble into a specific ribonucleoprotein complex called transamidosome that remains stable during the overall catalytic process, overview
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Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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cellular activity of AdTs only depends upon presence or lack of the mischarging amino acyl-tRNA synthetases
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