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6.3.5.6: asparaginyl-tRNA synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about asparaginyl-tRNA synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.6

Reaction

4-phosphooxy-L-aspartyl-tRNAAsn
+
NH3
=
L-asparaginyl-tRNAAsn
 +
phosphate

Synonyms

AdT, AsnRS, Asp-AdT, Asp-tRNAAsn amidotransferase, Asp/Glu-Adt, asparaginyl-transfer RNA synthetase, Asparaginyl-tRNA synthetase, aspartyl-tRNAAsn amidotransferase, GatCAB, glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, NRS, tRNA-dependent amidotransferase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.6 asparaginyl-tRNA synthase (glutamine-hydrolysing)

Cloned

Cloned on EC 6.3.5.6 - asparaginyl-tRNA synthase (glutamine-hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
canonical set of amidotransferase genes gatA, gatB and gatC is cloned and sequenced, the holoenzyme is overexpessed in Escherichia coli and exhibits both Asp-tRNAAsn and Glu-tRNAGln transamidation activities, the gatA, gatB and gatC genes are dispersed in the genome and encode polypeptides of 472, 470 and 90 amino acids, removal of the 44 carboxy-terminal amino acids of the GatA subunit only inhibits the Asp-AdT activity, but not the Glu-AdT activity
-
cloning of the gatC, gatA and gatB genes, situated in an operon-like manner, encoding the GatCAB amidotransferase and overexpression in Escherichia coli BL21-Codon Plus-TM
-
cloning of the gatCAB genes encoding the Asp/Glu-AdT and overexpression in Escherichia coli
-
cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL
-
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3), co-expression with the cytotoxic nondiscriminating aspartyl-tRNA synthetase, EC 6.1.1.23, which is toxic for the cells, rescues the toxicity, overview
-
gatCAB genes encode Asp-AdT
-
gatCAB genes encoding Glu/Asp-AdT are cloned and expressed in Escherichia coli
-
overexpression in Escherichia coli
-
presence of the gatCAB genes encoding Asp/Glu-AdT