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6.3.5.2: GMP synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about GMP synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.2

Reaction

ATP
+
XMP
 +
L-glutamine
 +
H2O
=
AMP
 +
diphosphate
 +
GMP
 +
L-glutamate

Synonyms

cgsA, di-GMP synthase A, EC 6.3.4.1, GATase, Glutamine amidotransferase, GMP synthase, GMP synthase [glutamine-hydrolyzing] subunit B, GMP synthetase, GMP synthetase (glutamine hydrolysing), GMPS, GUA, GuaA, Guanine monophosphate synthetase, Guanosine 5'-monophosphate synthetase, Guanosine 5-monophosphate synthetase, guanosine monophosphate synthetase, Guanosine monophosphate synthetase (glutamine-hydrolyzing), Guanylate synthetase, Guanylate synthetase (glutamine-hydrolyzing), More, PD0279, PH1346, Synthetase, guanylate, two-subunit-type GMP synthetase, Xanthosine 5'-phosphate amidotransferase, Xanthosine 5-monophosphate aminase, Xanthosine-5'-phosphate-ammonia ligase, Xanthosine-5'-phosphate:ammonia ligase (AMP-forming), Xanthosine-5'-phosphate:L-glutamine amido-ligase (AMP-forming), XMP aminase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.2 GMP synthase (glutamine-hydrolysing)

Engineering

Engineering on EC 6.3.5.2 - GMP synthase (glutamine-hydrolysing)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GP-1
-
psicofuranine, decoyinine and adenosine strongly inhibit wild type enzyme. Mutant enzyme GP-1 shows decreased inhibition with adenosine and psicofuranine, but inhibition by decoyinine does not vary, complete loss of inhibition in mutant MG-1
C95A
-
mutant defective in glutamine hydrolysis. The phenotype of ectopic coexpression of USP7 witheither S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development
S242L
-
mutant defective in ATP hydrolysis. The phenotype of ectopic coexpression of USP7 with either S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development
37W
-
the mutant shows severely reduced specific activity compared to the wild type enzyme
E383A
8fold decrease in glutaminase activity
H186A
30-50 fold decrease in Km value for glutamine
H186A/E383A
mutant exhibits poor solubility and basal glutaminase activity
H186E/E383H
interchange of the amino acids of the salt bridge, significant decrease in catalytic activity
W37A
-
the mutant shows slightly reduced specific activity compared to the wild type enzyme
C102A
-
totally devoid of glutamine-dependent activity. Kinetic constants for NH4Cl, ATP, and XMP obtained for the ammonia-dependent activity are similar to that of the wild-type enzyme
G388D
-
reduces the activity of GMP synthase Gua1 in budding yeast and the total G-nucleotide pool, leading to precipitous reductions in the GDP/GTP ratio and ATP level in vivo. G388D strongly reduces the rate of growth, impairs general protein synthesis, and derepresses translation of GCN4 mRNA, encoding a transcriptional activator of diverse amino acid biosynthetic enzymes. Although processing of pre-tRNAi Met and other tRNA precursors, and the aminoacylation of tRNAi Met are also strongly impaired in G388D cells, tRNAi Met-containing complexes with the macromolecular composition of the eIF2tRNAi Met.GTP complex and the multifactor complex required for translation initiation accumulate 10-fold in G388D cells and, to a lesser extent, in wild-type cells treated with 6-azauracil
additional information