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6.3.5.2: GMP synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about GMP synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.2

Reaction

ATP
+
XMP
 +
L-glutamine
 +
H2O
=
AMP
 +
diphosphate
 +
GMP
 +
L-glutamate

Synonyms

cgsA, di-GMP synthase A, EC 6.3.4.1, GATase, Glutamine amidotransferase, GMP synthase, GMP synthase [glutamine-hydrolyzing] subunit B, GMP synthetase, GMP synthetase (glutamine hydrolysing), GMPS, GUA, GuaA, Guanine monophosphate synthetase, Guanosine 5'-monophosphate synthetase, Guanosine 5-monophosphate synthetase, guanosine monophosphate synthetase, Guanosine monophosphate synthetase (glutamine-hydrolyzing), Guanylate synthetase, Guanylate synthetase (glutamine-hydrolyzing), More, PD0279, PH1346, Synthetase, guanylate, two-subunit-type GMP synthetase, Xanthosine 5'-phosphate amidotransferase, Xanthosine 5-monophosphate aminase, Xanthosine-5'-phosphate-ammonia ligase, Xanthosine-5'-phosphate:ammonia ligase (AMP-forming), Xanthosine-5'-phosphate:L-glutamine amido-ligase (AMP-forming), XMP aminase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.2 GMP synthase (glutamine-hydrolysing)

Cloned

Cloned on EC 6.3.5.2 - GMP synthase (glutamine-hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of ATPP/DD (a construct that contains the ATP-pyrophosphatase domain as well as the predicted dimerization domain), expression and purification of the corresponding protein, as both a His-tagged fusion protein (His-ATPP/DD) and as a non-fusion protein (NF-ATPP/DD). Both His-ATPP/DD and NF-ATPP/DD are active proteins, capable of catalyzing the conversion of XMP to GMP using exogenously added ammonia
construction of a plasmid for high level expression of XMP aminase in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) gold pRARE cells
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
gene guaA, the guaAB operon of Borrelia burgdorferi is present on plasmid cp26
-
genes ph1346 and ph1347, genetic structure of GMPS, overview. Expressionin Escherichia coli strain BL21(DE3)
isolation of the gene
overexpression in Escherichia coli
-
the Ecdysone receptor interacts biochemically and genetically with GMPS/USP7, co-expression of GST-tagged GMPS and USP7 in Escherichia coli strain BL21(DE3)
-