6.3.5.11: cobyrinate a,c-diamide synthase
This is an abbreviated version!
For detailed information about cobyrinate a,c-diamide synthase, go to the full flat file.
Reaction
2 ATP + + 2 L-glutamine + 2 H2O = + 2 phosphate + + 2 L-glutamate
Synonyms
CbiA, CobB
ECTree
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Application
Application on EC 6.3.5.11 - cobyrinate a,c-diamide synthase
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synthesis
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co-expression of the cobA gene from Propionibacterium freudenreichii and the cbiA, -C, -D, -E, -T, -F, -G, -H, -J, -K, -L, and -P genes from Salmonella enterica serovar typhimurium in Escherichia coli result in the production of cobyrinic acid a,c-diamide. Strains that have neither cbiP nor cbiA synthesized 1-desmethylcobyrinic acid even in the presence of cbiD, suggesting that CbiA and CbiP are necessary for CbiD activity