Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.5.1: NAD+ synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about NAD+ synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.1

Reaction

ATP
+
deamido-NAD+
+
L-glutamine
+
H2O
=
AMP
+
diphosphate
+
NAD+
+
L-glutamate

Synonyms

abNadE, General stress protein 38, glutamine-dependent NAD synthetase, glutamine-dependent NAD+ synthetase, glutamine-dependent NadEGln, GSP38, Hsero_1894, mtuNadE, NAD synthetase (glutamine), NAD(+) synthase [glutamine-hydrolyzing], NAD+ synthase, NAD+ synthetase, NAD+ synthetase (glutamine-hydrolysing), NadE-679, NadE-738, NadE1, NADS, nic13, Nicotinamide adenine dinucleotide synthetase (glutamine), nicotinamide adenine dinucleotide synthetase enzyme, Nitrogen-regulatory protein, Qns1, Sporulation protein outB, Synthetase, nicotinamide adenine dinucleotide (glutamine)

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.1 NAD+ synthase (glutamine-hydrolysing)

Purification

Purification on EC 6.3.5.1 - NAD+ synthase (glutamine-hydrolysing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutant enzymes expressed in Escherichia coli
-
partial
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant NAD+ synthase, Ni2+ chelate resin affinity chromatography
-
recombinant NadE-738 and NadE-679, Ni2+-nitrilotriacetic acid agarose, gel filtration
-
using Ni-NTA chromatography