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6.3.4.3: formate-tetrahydrofolate ligase

This is an abbreviated version!
For detailed information about formate-tetrahydrofolate ligase, go to the full flat file.

Word Map on EC 6.3.4.3

Reaction

ATP
+
formate
 +
tetrahydrofolate
=
ADP
 +
phosphate
 +
10-formyltetrahydrofolate

Synonyms

10-CHO-THF synthase, 10-formyl-THF synthetase, 10-Formyltetrahydrofolate synthetase, 10-formylTHF synthetase, ADE3, C(1)-tetrahydrofolate synthase, C1 synthase, C1-tetrahydrofolate synthase, C1-tetrahydrofolate synthetase, C1-THF synthase, C1-THFS, CpeFhs, DCS, dehydrogenasse-cyclohydrolase-synthetase, FHS, formate-tetrahydrofolate ligase, formate:tetrahydrofolate ligase (ADP-forming), formyl tetrahydrofolate synthetase, Formyl-THF synthetase, Formyltetrahydrofolate synthetase, ftfL, FTHFS, FTL, methylenetetrahydrofolate dehydrogenase 1-like, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase enzyme, MIS1, MTHFD, MTHFD1, MTHFD1L, MTHFDIL, N10-formyltetrahydrofolate synthetase, NAD-dependent methylenetetrahydrofolate dehydrogenase, Synthetase, formyl tetrahydrofolate, Tetrahydrofolate formylase, Tetrahydrofolic formylase, THF synthase, THFS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.3 formate-tetrahydrofolate ligase

Cloned

Cloned on EC 6.3.4.3 - formate-tetrahydrofolate ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deletion of the MIS1 gene has little effect
DNA sequence determination and analysis
expressed in CHO cells and Escherichia coli
expressed in Escherichia coli
-
expressed in Escherichia coli strain Y1
expression in CHO cells and Saccharomyces cerevisiae
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli, localization of the interdomain region of the trifunctional enzyme by site-directed mutagenesis
-
expression of the mitochondrial isozyme as maltose-binding protein fusion protein in Escherichia coli, overexpression in yeast
-
gene DKFZp586G1517, DNA and amino acid sequence determination and analysis, overexpression of mitochondrial enzyme in HEK-293 cells, unlabeled or FLAG-tagged, stimulates cell growth
gene fhs, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Rosetta
gene FTHFS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic clusters, overview. Cloning and expression in Escherichia coli
gene fths, DNA and amino acid sequence determination and analysis, quantitative expression analysis by real-time PCR, phylogenetic analysis, cloning and expression in Escherichia coli strain JM109
overexpression of FTL using the multicopy episomal vector pXNG4-FTL
-
the wild type and R653Q mutant pBKeDCS constructs are transformed into Escherichia coli BL21 DE3 to express the full-length protein