6.3.4.22: tRNAIle2-agmatinylcytidine synthase

This is an abbreviated version!
For detailed information about tRNAIle2-agmatinylcytidine synthase, go to the full flat file.

Reaction

Synonyms

AF2259, TiaS, tRNAIle-2-agmatinylcytidine synthetase, tRNAIle-agm2C synthetase, tRNAIle-agmatidine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.4 Other carbon-nitrogen ligases

                6.3.4.22 tRNAIle2-agmatinylcytidine synthase

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Results	in table
1809	AA Sequence
3	Cloned(Commentary)
2	Crystallization (Commentary)
2	General Information
2	IC50 Value
2	Inhibitors
3	kcat/KM [mM/s]
3	KM Value [mM]
2	Metals/Ions
2	Natural Substrates/ Products (Substrates)
5	Organism
3	PDB ID
1	pH Optimum
1	Posttranslational Modification
28	Protein Variants
4	Purification (Commentary)
1	Reaction
5	Reference
4	Substrates and Products (Substrate)
16	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 6.3.4.22 - tRNAIle2-agmatinylcytidine synthase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + ?

2 entries

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate

2 entries

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + ?

Archaeoglobus fulgidus

O28025

-

705848

-

-

?

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + ?

Pyrococcus horikoshii

O59476

-

705848

-

-

?

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate

Archaeoglobus fulgidus

O28025

the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle

720531

-

-

?

ATP + agmatine + [tRNAIle2]-cytidine34

[tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate

Archaeoglobus fulgidus

O28025

the precursor form of tRNAIle bearing an unmodified cytidine at the wobble position (C34) is aminoacylated with methionine (Met) and decodes the AUG codon.The Thr18-Cyt34 kinase domain first hydrolyzes ATP into AMP and diphosphate, then phosphorylates the C2 position of cytidine34 with the gamma-phosphate. Next, the amino group of agmatine attacks this position to release the phosphate and form 2-agmatinylcytidine. The Thr18-Cyt34 kinase domain also autophosphorylates the Thr18 of the enzyme, which may be involved in 2-agmatinylcytidine formation. Catalytic efficiency (kcat/Km) with GTP, UTP or CTP as substrate is less than 1% compared to ATP

720531

-

-

?