6.3.4.20: 7-cyano-7-deazaguanine synthase

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For detailed information about 7-cyano-7-deazaguanine synthase, go to the full flat file.

Reaction

Synonyms

7-cyano-7-carbaguanine synthase, preQ0 synthase, queC

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.4 Other carbon-nitrogen ligases

6.3.4.20 7-cyano-7-deazaguanine synthase

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Results	in table
22464	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
10	General Information
5	Metals/Ions
7	Natural Substrates/ Products (Substrates)
7	Organism
5	Pathway
2	pH Optimum
1	pH Range
1	pI Value
2	Protein Variants
3	Purification (Commentary)
1	Reaction
7	Reference
14	Substrates and Products (Substrate)
1	Subunits
10	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
5	Temperature Stability [°C]

Purification

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Purification on EC 6.3.4.20 - 7-cyano-7-deazaguanine synthase

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Geobacillus kaustophilus

A0A0N9HMW6

744701

recombinant His-tagged QueC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Bacillus subtilis

717240

recombinant QueC from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration

Bacillus subtilis

O31675

718371