6.3.4.20: 7-cyano-7-deazaguanine synthase
This is an abbreviated version!
For detailed information about 7-cyano-7-deazaguanine synthase, go to the full flat file.
Reaction
Synonyms
7-cyano-7-carbaguanine synthase, preQ0 synthase, queC
ECTree
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Engineering
Engineering on EC 6.3.4.20 - 7-cyano-7-deazaguanine synthase
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additional information
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in vitro preparation of the deazapurine nucleoside, preQ0, by the successive action of the four involved enzymes, i.e. Escherichia coli QueD, Bacillus subtilis QueE and QueC, and Streptomyces rimosus ToyM, cloning and expression in Escherichia coli strain BL21(DE3), overview
additional information
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the expression of QueC from a plasmid-borne copy confers a Q+ phenotype to enzyme-deficient mutant strain Escherichia coli B105, mapping of the transposon insertion site, overview. Generation of a knockout of queC in Escherichia coli strain JE10651, a queA mutant