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6.3.4.14: biotin carboxylase

This is an abbreviated version!
For detailed information about biotin carboxylase, go to the full flat file.

Word Map on EC 6.3.4.14

Reaction

ATP
+
[biotin carboxyl-carrier protein]-biotin-N6-L-lysine
+
hydrogencarbonate-
=
ADP
+
phosphate
+
[biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine

Synonyms

ACC, AccA, AccBC, AccC, BC, biotin carboxylase, biotin carboxylase (component of acetyl CoA carboxylase), biotinoyl domain of acetyl-CoA carboxylase, BirA, Carboxylase, biotin, More, PC-beta

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.14 biotin carboxylase

Purification

Purification on EC 6.3.4.14 - biotin carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hybrid dimers composed of one subunit having an active site mutation and a second with a wild-type active site
-
native and E288K mutant enzyme
-
Ni-NTA column chromatography
nickel affinity chromatography and gel filtration
partial
-
partial purification
-
recombinant biotin carboxylase domain of pyruvate carboxylase
-
recombinant enzyme
-
recombinant His-tagged biotinoyl domain from Escherichia coli strain AVB101 by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli srrain BL21-AI by nickel affinity and anion exchange chromatography, and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21-AI by nickel affinity chromatography and gel filtration
-
recombinant wild-type and mutant His-tagged biotinoyl domains from Escherichia coli strain AVB101 by nickel affinity chromatography and gel filtration
-
wild-type and mutant enzymes
-
wild-type and mutant, overexpressed BC
-