6.3.4.10: biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase
This is an abbreviated version!
For detailed information about biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase, go to the full flat file.
Word Map on EC 6.3.4.10
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6.3.4.10
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biotinylation
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carboxylases
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biotin-dependent
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histone
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biotinidase
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acidosis
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biotin-responsive
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multivitamin
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aciduria
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biotinyl-5\'-amp
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biotin-deficient
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apocarboxylases
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methylcitrate
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3-hydroxyisovaleric
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hyperammonemia
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tokodaii
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3-methylcrotonyl-coa
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biotin-binding
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diagnostics
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medicine
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analysis
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synthesis
- 6.3.4.10
-
biotinylation
- carboxylases
-
biotin-dependent
- histone
- biotinidase
- acidosis
-
biotin-responsive
-
multivitamin
- aciduria
-
biotinyl-5\'-amp
-
biotin-deficient
- apocarboxylases
-
methylcitrate
-
3-hydroxyisovaleric
-
hyperammonemia
- tokodaii
- 3-methylcrotonyl-coa
-
biotin-binding
- diagnostics
- medicine
- analysis
- synthesis
Reaction
Synonyms
biotin protein ligase, Biotin-propionyl coenzyme A carboxylase synthetase, Biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] synthetase, BPL, HCS, HLCS, Holocarboxylase synthetase, Propionyl coenzyme A holocarboxylase synthetase, Synthetase, biotin-propionyl coenzyme A carboxylase
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Application
Application on EC 6.3.4.10 - biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase
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analysis
diagnostics
medicine
synthesis
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96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity by biotinylation of the polypeptide p67, which comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669, using IRDye-streptavidin and infrared spectroscopy. The minimal concentration of recombinant HCS that can be detected by this assay is less than 1.08 nmol/l. Jurkat cells contain 0.14 U of HCS activity [in micromol of biotinylated p67 formed/(nmol/l HCS h)] in 400 microg of total protein
analysis
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fusion of full-length HCS to DNA adenine methyltransferase Dam and subsequent transfection into breast cancer cells MCF-7 and normal breast cells MCF-10A for identification of chromatin binding sites
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the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
diagnostics
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the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
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HCS deficiency results in biotin-responsive multiple carboxylase deficiency
medicine
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identifaction of patients with holocarboxylase synthetase deficiency by newborn screening
site-directed antibody immobilization by fusing biotin carboxyl carrier protein BCCP to the IgG-binding domain of Protein A, and the resulting fusion protein is immobilized on the biotin protein ligase-modified gold surface of the sensor chip for quartz crystal microbalance through complexation between BCCP and biotin protein ligase. The layer of the IgG-binding domain is successfully captured the antibody, and the antibody retains high antigen-binding capability
synthesis
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site-directed antibody immobilization by fusing biotin carboxyl carrier protein BCCP to the IgG-binding domain of Protein A, and the resulting fusion protein is immobilized on the biotin protein ligase-modified gold surface of the sensor chip for quartz crystal microbalance through complexation between BCCP and biotin protein ligase. The layer of the IgG-binding domain is successfully captured the antibody, and the antibody retains high antigen-binding capability
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