6.3.3.3: dethiobiotin synthase

This is an abbreviated version!
For detailed information about dethiobiotin synthase, go to the full flat file.

Word Map on EC 6.3.3.3

6.3.3.3

7,8-diaminopelargonic

aminotransferase

dapas

penultimate

ureido

carbamate

diaminopelargonic

Reaction

Synonyms

BIO3, BioD, BioD1, Desthiobiotin synthetase, Dethiobiotin synthase, Dethiobiotin synthetase, DTB synthetase, DTBS, Synthetase, dethiobiotin

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.3 Cyclo-ligases

6.3.3.3 dethiobiotin synthase

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Results	in table
23520	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
1	Cofactor
12	Crystallization (Commentary)
9	IC50 Value
28	Inhibitors
3	Ki Value [mM]
43	KM Value [mM]
1	Localization
9	Metals/Ions
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
42	Organism
6	Pathway
158	PDB ID
2	pH Optimum
6	Protein Variants
9	Purification (Commentary)
7	Reaction
2	Reaction Type
42	Reference
2	Source Tissue
6	Specific Activity [micromol/min/mg]
2	Storage Stability
86	Substrates and Products (Substrate)
6	Subunits
16	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
28	Turnover Number [1/s]

Cloned

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Cloned on EC 6.3.3.3 - dethiobiotin synthase

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5 entries

cloning of the biotin biosynthetic operon

Bacillus subtilis

633

expression in Escherichia coli

Mycobacterium tuberculosis

714208

gene bio3, alleles bio1-3, bio2-3, and bio3-3, DNA and amino acid sequence determination and analysis and genotyping, encoded with gene bio1 in a bifunctional locus that catalyzes two sequential reactions in the same metabolic pathway via differential splicing, separate BIO3 and BIO1 transcripts and two different types of chimeric BIO3-BIO1 transcripts are produced. One of the fused transcripts is monocistronic and encodes a bifunctional fusion protein. A splice variant is bicistronic, with distinct but overlapping reading frames, overview

nucleotide sequence

overexpression in Escherichia coli

Escherichia coli

1339