6.3.3.2: 5-formyltetrahydrofolate cyclo-ligase

This is an abbreviated version!
For detailed information about 5-formyltetrahydrofolate cyclo-ligase, go to the full flat file.

Reaction

Synonyms

5,10-methenyl-tetrahydrofolate synthetase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-Methenyltetrahydropteroylglutamate synthetase, 5-CHO-THF cycloligase, 5-FCL, 5-Formyltetrahydrofolate cyclodehydrase, CH+-THF synthetase, Fau1p, Formyltetrahydrofolic cyclodehydrase, Methenyl THFS, Methenyl-THF synthetase, Methenyltetrahydrofolate synthetase, More, MTHFD1, MTHFS, N5-Formyltetrahydrofolic acid cyclodehydrase, Synthetase, methenyltetrahydrofolate, ygfA

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.3 Cyclo-ligases

                6.3.3.2 5-formyltetrahydrofolate cyclo-ligase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
4	Activating Compound
36539	AA Sequence
4	Application
1	CAS Registry Number
19	Cloned(Commentary)
16	Cofactor
4	Crystallization (Commentary)
470	Disease/ Diagnostics
1	Expression
5	General Information
4	General Stability
6	IC50 Value
49	Inhibitors
18	Ki Value [mM]
76	KM Value [mM]
15	Localization
27	Metals/Ions
19	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
48	Organism
1	Oxidation Stability
7	Pathway
12	PDB ID
11	pH Optimum
2	pH Range
2	pH Stability
38	Protein Variants
20	Purification (Commentary)
9	Reaction
7	Reaction Type
48	Reference
53	Source Tissue
20	Specific Activity [micromol/min/mg]
3	Storage Stability
98	Substrates and Products (Substrate)
15	Subunits
66	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
4	Temperature Stability [°C]
26	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

araATP + 5-formyltetrahydrofolate

araADP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

ATP + (6R,S)-5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

-

-

1319

-

-

?

ATP + (6R,S)-5-formyltetrahydropteroyl-Glu

?

Homo sapiens

-

-

1329, 1330

-

-

?

ATP + (6S)-5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

2 entries

ATP + (6S)-5-formyltetrahydropteroyl-pentaglutamate

?

Homo sapiens

-

-

1319, 1329, 1330

-

-

?

ATP + 5-formyltetrahydrofolate

?

4 entries

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

15 entries

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

19 entries

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

22 entries

ATP + 5-formyltetrahydrofolate-pentaglutamate

ADP + phosphate + 5,10-methylenetetrahydrofolate-pentaglutamate

Arabidopsis thaliana

Q8L539

preferred substrate to the monoglutamyl form

652326

-

652326

ir

ATP + 5-formyltetrahydrohomofolate

?

Oryctolagus cuniculus

-

-

1331

-

-

?

ATP + 5-formyltetrahydropteroyl-pentaglutamate

?

2 entries

ATPgammaS + 5-formyltetrahydrofolate

ADPgammaS + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

CTP + 5-formyltetrahydrofolate

CDP + phosphate + 5,10-methenyltetrahydrofolate

Oryctolagus cuniculus

-

CTP in form of MgCTP2-

1321

-

-

?

CTP + 5-formyltetrahydrofolate

CDP + phosphate + 5,10-methylenetetrahydrofolate

3 entries

dATP + 5-formyltetrahydrofolate

dADP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

dTTP + 5-formyltetrahydrofolate

dTDP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

GTP + 5-formyltetrahydrofolate

GDP + phosphate + 5,10-methylenetetrahydrofolate

2 entries

ITP + 5-formyltetrahydrofolate

IDP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

UTP + 5-formyltetrahydrofolate

UDP + phosphate + 5,10-methenyltetrahydrofolate

Oryctolagus cuniculus

-

UTP in form of MgUTP2-

1321

-

-

?

UTP + 5-formyltetrahydrofolate

UDP + phosphate + 5,10-methylenetetrahydrofolate

2 entries

additional information

?

-

6 entries

ATP + (6S)-5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus

Q9D110

-

663298

-

-

?

ATP + (6S)-5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus 129/Sv

Q9D110

-

663298

-

-

?

ATP + 5-formyltetrahydrofolate

?

Homo sapiens

-

-

1327

-

-

?

ATP + 5-formyltetrahydrofolate

?

Homo sapiens

-

obligatory initial metabolic step in the intracellular conversion of 5-formyltetrahydrofolate to other reduced folates

1326

-

-

?

ATP + 5-formyltetrahydrofolate

?

Oryctolagus cuniculus

-

it is proposed that methenyltetrahydrofolate synthetase is a salvage enzyme which converts the nonenzymatically formed and nonmetabolically active 5-formyltetrahydropteroyl-(Glu)n back into the one-carbon donor folate pool. It is also possible that methenyltetrahydrofolate synthetase is part of a regulation system in the cell in which its substrate 5-formyl-tetrahydropteroyl-(Glu)n plays a role as a regulator of one-carbon metabolism by inhibiting other folate requiring enzymes

1328

-

-

?

ATP + 5-formyltetrahydrofolate

?

Rattus norvegicus

-

key enzymatic reaction in the pathway by which 5-formyltetrahydrofolate enters the folate coenzyme pool

1327

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Arabidopsis thaliana

-

-

662384

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Arabidopsis thaliana

-

recycling reaction

662384

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Bacillus anthracis

Q81LX0

-

671158

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Bacillus anthracis BA4489

Q81LX0

-

671158

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Homo sapiens

-

-

674740

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Homo sapiens

-

important reaction in one-carbon donor recycling

662472

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Homo sapiens

-

i.e. 5-FTHF or leucovirin

662472

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus

-

-

671613

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus

-

-

671619

-

-

ir

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus

Q9D110

the enzyme affects intracellular folate concentrations by accelerating folate degradation

663298

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mus musculus 129/Sv

Q9D110

the enzyme affects intracellular folate concentrations by accelerating folate degradation

663298

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mycoplasma pneumoniae

-

-

663356, 663363

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Mycoplasma pneumoniae

P75430

-

694983

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Oryctolagus cuniculus

-

-

671613

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + 5,10-methenyltetrahydrofolate + phosphate

Saccharomyces cerevisiae

-

-

675367

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Arabidopsis thaliana

Q8L539

-

652326

-

652326

ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

-

-

701907

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

P49914

-

703027

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

-

enzyme prefers the polyglutamate forms of the substrate compared to the monoglutamate form

652430

-

652430

ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

-

enzyme plays an important role in one-carbon metabolism, overview about folate catabolism

652430

-

652430

ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Homo sapiens

P49914

folate binds in a spherical pocket, substrate binding and active site structure, structure-function analysis, detailed overview

703027

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Lacticaseibacillus casei

-

ATP in form of MgATP2-

1320, 1322

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mus musculus

-

-

652430

-

652430

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mus musculus

-

-

701907

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mus musculus

Q9D110

ATP in form of MgATP2-

663298

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mus musculus 129/Sv

Q9D110

ATP in form of MgATP2-

663298

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mycoplasma pneumoniae

-

-

728751

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Mycoplasma pneumoniae

-

ATP in form of MgATP2-

663363

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Oryctolagus cuniculus

-

-

649293

-

649293

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Oryctolagus cuniculus

-

enzyme plays a central role in the regulation of folate-dependent, one-carbon metabolism

649293

-

649293

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Oryctolagus cuniculus

-

ATP in form of MgATP2-

1321, 1328, 1331

-

-

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Pisum sativum

-

-

652326

-

652326

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Saccharomyces cerevisiae

-

-

652260

-

652260

?, ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methenyltetrahydrofolate

Solanum lycopersicum

Q8LKF6

-

652326

-

652326

ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Arabidopsis thaliana

Q8L539

-

652326

-

652326

ir

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Bos taurus

-

-

1332

-

1332

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Cricetulus griseus

-

-

1327

-

1327

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Gallus gallus

-

-

1320

-

1320

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

-

1326

-

1326

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

-

1327

-

1327

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

-

1329

-

1329

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

-

1330

-

1330

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

no reverse reaction detected

1319

-

1319

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Homo sapiens

-

(6R,S)-5-formyltetrahydrofolate, (6S)-5-formyltetrahydrofolate

1319

-

1319

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1320

-

1320

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

1322

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Mus musculus

-

-

1320

-

1320

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1320

-

1320

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1321

-

1321

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1331

-

1331

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

i.e. leucovorin, ir

1325

-

1325

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

i.e. leucovorin, ir

1328

-

1328

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Ovis aries

-

-

1320

-

1320

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Ovis aries

-

-

1323

-

1323

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Ovis aries

-

-

1324

-

1324

?

ATP + 5-formyltetrahydrofolate

ADP + phosphate + 5,10-methylenetetrahydrofolate

Rattus norvegicus

-

-

1327

-

1327

?

ATP + 5-formyltetrahydropteroyl-pentaglutamate

?

Homo sapiens

-

-

1319, 1329

-

-

?

ATP + 5-formyltetrahydropteroyl-pentaglutamate

?

Oryctolagus cuniculus

-

-

1331

-

-

?

CTP + 5-formyltetrahydrofolate

CDP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

CTP + 5-formyltetrahydrofolate

CDP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1321

-

-

?

CTP + 5-formyltetrahydrofolate

CDP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

as effective as ATP

1328, 1331

-

-

?

GTP + 5-formyltetrahydrofolate

GDP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

GTP + 5-formyltetrahydrofolate

GDP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1321

-

-

?

UTP + 5-formyltetrahydrofolate

UDP + phosphate + 5,10-methylenetetrahydrofolate

Lacticaseibacillus casei

-

-

1322

-

-

?

UTP + 5-formyltetrahydrofolate

UDP + phosphate + 5,10-methylenetetrahydrofolate

Oryctolagus cuniculus

-

-

1321

-

-

?

additional information

?

-

Arabidopsis thaliana

Q8L539

enzyme is specific for the 6S-isomer

652326

-

652326

?

additional information

?

-

Homo sapiens

-

increased MTHFS expression affects the efficacy of the glycinamide ribonucleotide formyltransferase inhibitor LY309887, overview. SH-SY5Y neuroblastoma with increased MTHFS expression display a 4fold resistance to the GARFT inhibitor LY309887, but do not exhibit resistance to the thymidylate synthase inhibitor Pemetrexed.

701907

-

-

?

additional information

?

-

Mus musculus

Q9D110

disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects

663298

-

-

?

additional information

?

-

Mus musculus

-

disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects

663298

-

-

?

additional information

?

-

Mus musculus

-

5-formyltetrahydrohomofolate, 10-formyltetrahydrohomofolate, and 5-formyl,10-methyltetrahydrofolate are no substrates

671619

-

-

?

additional information

?

-

Mus musculus 129/Sv

Q9D110

disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects

663298

-

-

?