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6.3.3.2: 5-formyltetrahydrofolate cyclo-ligase

This is an abbreviated version!
For detailed information about 5-formyltetrahydrofolate cyclo-ligase, go to the full flat file.

Word Map on EC 6.3.3.2

Reaction

ATP
+
5-formyltetrahydrofolate
=
ADP
 +
phosphate
 +
5,10-methenyltetrahydrofolate

Synonyms

5,10-methenyl-tetrahydrofolate synthetase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-Methenyltetrahydropteroylglutamate synthetase, 5-CHO-THF cycloligase, 5-FCL, 5-Formyltetrahydrofolate cyclodehydrase, CH+-THF synthetase, Fau1p, Formyltetrahydrofolic cyclodehydrase, Methenyl THFS, Methenyl-THF synthetase, Methenyltetrahydrofolate synthetase, More, MTHFD1, MTHFS, N5-Formyltetrahydrofolic acid cyclodehydrase, Synthetase, methenyltetrahydrofolate, ygfA

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.3 Cyclo-ligases
                6.3.3.2 5-formyltetrahydrofolate cyclo-ligase

Engineering

Engineering on EC 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D154A
site-directed mutagenesis, the mutant shows 0.9% of the wild-type catalytic activity
E225Stop
mutation identified in a patient suffering MTHFD1 deficiency
E61A
site-directed mutagenesis, the mutant shows 8.6% of the wild-type catalytic activity
F55A
site-directed mutagenesis, the mutant shows 53.6% of the wild-type catalytic activity
F85A
site-directed mutagenesis, the mutant shows 72.3% of the wild-type catalytic activity
K10A
site-directed mutagenesis, the mutant shows 7.5% of the wild-type catalytic activity
K150A
site-directed mutagenesis, the mutant shows wild-type catalytic activity
M58A
site-directed mutagenesis, the mutant shows 121.6% of the wild-type catalytic activity
M90A
site-directed mutagenesis, the mutant shows 49.2% of the wild-type catalytic activity
R145A
site-directed mutagenesis, the mutant shows 2.3% of the wild-type catalytic activity
R148A
site-directed mutagenesis, the mutant shows 30.7% of the wild-type catalytic activity
R14A
site-directed mutagenesis, the mutant shows 12.9% of the wild-type catalytic activity
S49F
mutation identified in a patient suffering MTHFD1 deficiency
T296I
mutation identified in a patient suffering MTHFD1 deficiency
W109A
site-directed mutagenesis, the mutant shows 39.7% of the wild-type catalytic activity
Y152A
site-directed mutagenesis, the mutant shows 24.9% of the wild-type catalytic activity
Y153A
site-directed mutagenesis, the mutant shows 3.2% of the wild-type catalytic activity
Y83A
site-directed mutagenesis, the mutant shows wild-type catalytic activity
Y151
-
able to use 5-formyltetrahydrohomofolate as a substrate
Y152A
-
increased turnover rate compared to the wild type enzyme
Y152F
-
decreased turnover rate compared to the wild type enzyme
D124A
-
inactive
D151A
-
the mutant shows increased turnover number compared to the wild type enzyme
D154A
-
inactive
D81A
-
the mutant shows reduced turnover number compared to the wild type enzyme
K120A
K3A
-
the mutant shows strongly reduced turnover number compared to the wild type enzyme
K3D/D151K
-
the mutant shows strongly reduced turnover number compared to the wild type enzyme
K3D/D154 K
-
inactive
R115A
the mutant shows no activity
R125A
-
the mutant shows reduced turnover number compared to the wild type enzyme
R7A
-
the mutant shows severely reduced turnover number compared to the wild type enzyme
W153A
-
the mutant shows reduced turnover number compared to the wild type enzyme
additional information