6.3.3.2: 5-formyltetrahydrofolate cyclo-ligase
This is an abbreviated version!
For detailed information about 5-formyltetrahydrofolate cyclo-ligase, go to the full flat file.
Word Map on EC 6.3.3.2
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6.3.3.2
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mthfr
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methylenetetrahydrofolate
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one-carbon
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homocysteine
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folate-dependent
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slc19a1
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10-formyltetrahydrofolate
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trifunctional
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cyclohydrolase
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5,10-methylenetetrahydrofolate
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remethylation
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folate-mediated
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folate-related
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1-carbon
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megaloblastic
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periconceptional
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folate-metabolizing
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analysis
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pharmacology
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medicine
- 6.3.3.2
- mthfr
- methylenetetrahydrofolate
-
one-carbon
- homocysteine
-
folate-dependent
-
slc19a1
- 10-formyltetrahydrofolate
-
trifunctional
-
cyclohydrolase
- 5,10-methylenetetrahydrofolate
-
remethylation
-
folate-mediated
-
folate-related
-
1-carbon
-
megaloblastic
-
periconceptional
-
folate-metabolizing
- analysis
- pharmacology
- medicine
Reaction
Synonyms
5,10-methenyl-tetrahydrofolate synthetase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-Methenyltetrahydropteroylglutamate synthetase, 5-CHO-THF cycloligase, 5-FCL, 5-Formyltetrahydrofolate cyclodehydrase, CH+-THF synthetase, Fau1p, Formyltetrahydrofolic cyclodehydrase, Methenyl THFS, Methenyl-THF synthetase, Methenyltetrahydrofolate synthetase, More, MTHFD1, MTHFS, N5-Formyltetrahydrofolic acid cyclodehydrase, Synthetase, methenyltetrahydrofolate, ygfA
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Crystallization
Crystallization on EC 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase
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cocrystallization with folinate, ATP and Mg2+, sitting drop vapour diffusion method using 25% polyethylene glycol 3350, 100 mM bis-Tris pH 5.5
Q81LX0
purified recombinant wild-type MTHFS in a binary complex with ADP, with the N5-iminium phosphate reaction intermediate, or as an enzyme-product complex, hanging drop method at 16°C, 600 nl protein solution, with or without 5 mM 5-formyltetrahydrofolate and 5 mM ATP, are equilibrated against 0.05 ml of reservoir solution containing 100 mM HEPES, pH 6.6, 20 mM MgCl2, 20 mM NiCl2, and 20% w/v PEG 3350, 2 days, X-ray diffraction structure determination and analysis
purified recombinant enzyme complexed with ADP, phosphate, and 5-formyltetrahydrofolate, hanging drop vapour diffusion method, 20°C, 30 mg/ml protein with 20% PEG 4000, 5 mM ATP, and 10 mM 5-formylTHF, flash freezing of all crystals in 20% PEG 4000 and 30% ethylene glycol, X-ray diffraction structure determination and analysis at 2.5 A resolution, crystallization of the enzyme with ATP analogues ATP-gamma-S, AMP-PNP, or AMP-PCP is not successful
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purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution
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