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6.3.3.2: 5-formyltetrahydrofolate cyclo-ligase

This is an abbreviated version!
For detailed information about 5-formyltetrahydrofolate cyclo-ligase, go to the full flat file.

Word Map on EC 6.3.3.2

Reaction

ATP
+
5-formyltetrahydrofolate
=
ADP
+
phosphate
+
5,10-methenyltetrahydrofolate

Synonyms

5,10-methenyl-tetrahydrofolate synthetase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-Methenyltetrahydropteroylglutamate synthetase, 5-CHO-THF cycloligase, 5-FCL, 5-Formyltetrahydrofolate cyclodehydrase, CH+-THF synthetase, Fau1p, Formyltetrahydrofolic cyclodehydrase, Methenyl THFS, Methenyl-THF synthetase, Methenyltetrahydrofolate synthetase, More, MTHFD1, MTHFS, N5-Formyltetrahydrofolic acid cyclodehydrase, Synthetase, methenyltetrahydrofolate, ygfA

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.3 Cyclo-ligases
                6.3.3.2 5-formyltetrahydrofolate cyclo-ligase

Cloned

Cloned on EC 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from brain cDNA library, expression of the His-tagged enzyme in Escherichia coli strain BL21
cytsolic enzyme
-
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, yield of purified recombinant enzyme depends on the growth conditions, M9 media is referred, overview
DNA sequence determination and analysis, expression in Escherichia coli BL21 as full length or truncated protein, the latter lacking the mitochondrial targeting sequence, functional complementation of enzyme deficient yeast fau1 mutant, yeast strain WHY1.3.1
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as full length or truncated protein, the latter lacking the mitochondrial targeting sequence
DNA sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli (strain Rosetta pLysS)
Q81LX0
expressed in Escherichia coli BL21 Star cells and MCF-7 cells
expressed in Escherichia coli BL21(DE3)/pSJS1244 cells
-
expression in Escherichia coli
expression in MCF-7 mammary adenocarcinoma cell line and in SH-SY5Y neuroblastoma cell line
-
expression of truncation mutant comprising residues 6-202 in Escherichia coli strain BL21(DE3)
-
gene FAU1, orf YER183c, functional expression as His-tagged enzyme in Escherichia coliBL21(DE3)
-
insertional mutant DNA sequence determination and analysis
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
wild type and mutant enzymes are expressed in Escherichia coli strain BL21(DE3)/pSJS1244