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6.3.2.43: [amino group carrier protein]-L-2-aminoadipate ligase

This is an abbreviated version!
For detailed information about [amino group carrier protein]-L-2-aminoadipate ligase, go to the full flat file.

Reaction

ATP
+
an [amino group carrier protein]-C-terminal-L-glutamate
+
L-2-aminoadipate
=
ADP
+
phosphate
+
an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutyl)-L-glutamine

Synonyms

alpha-aminoadipate-lysW ligase lysX, LysX, More, RimK-related lysine biosynthesis protein, Saci_0754, Tk0278, TkLysX/ArgX, TtLysX, [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming), [lysine-biosynthesis-protein LysW]-L-2-aminoadipate ligase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.43 [amino group carrier protein]-L-2-aminoadipate ligase

Purification

Purification on EC 6.3.2.43 - [amino group carrier protein]-L-2-aminoadipate ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by heat treatment at 80°C for 20 min and nickel affinity chromatography. Recombinant nontagged enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 80°C for 20 min, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and gel filtration