6.3.2.43: [amino group carrier protein]-L-2-aminoadipate ligase
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For detailed information about [amino group carrier protein]-L-2-aminoadipate ligase, go to the full flat file.
Reaction
Synonyms
alpha-aminoadipate-lysW ligase lysX, LysX, More, RimK-related lysine biosynthesis protein, Saci_0754, Tk0278, TkLysX/ArgX, TtLysX, [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming), [lysine-biosynthesis-protein LysW]-L-2-aminoadipate ligase
ECTree
6.3.2.43 [amino group carrier protein]-L-2-aminoadipate ligaseAdvanced search results
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results (Crystallization
Crystallization on EC 6.3.2.43 - [amino group carrier protein]-L-2-aminoadipate ligase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
in presence of carrier protein LysW and AMP-PNP, MgSO4, and L-2-aminoadipate or glutamate, to 2.18 A resolution
purified recombinant nontagged enzyme TkLysX/ArgX in complex with [LysW]-C-terminal-N-(1,5-dicarboxypentan-1-yl)-L-glutamine ((TkLysX/ArgXx02LysW-gamma-AAA)), which corresponds to the structure of the post-reaction state of TkLysX/Arg, hanging drop vapor diffusion method, mixing of 7.5 mg/ml protein and 2.5 mg/ml TkLysW in 10 mM AMP-PNP, 10 mM AAA, or 50 mM glutamate and 10 mM MgSO4 with precipitation solution containing containing 20% v/v 2-methyl-2,4-pentanediol, 6.6% w/v PEG-3350, 0.066 M imidazole, pH 6.5, and 0.132 M ammonium sulfate, 20°C, X-ray diffraction structure determination and analysis at 2.18 A resolution. Molecular replacement. The asymmetric unit contains two TkLysX/ArgX tetramers, two intact TkLysW monomers each with one zinc atom, four partial TkLysW, eight ADP molecules, five phosphate ions, two sulfate ions, nine magnesium atoms, one free AAA molecule, and 628 water molecules. Good crystals containing TkLysW-gamma-Glu are not obtained
crystal structures of LysX and its complex with ADP at 2.0 A and 2.38 A resolutions
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hanging-drop vapour-diffusion technique in two different space groups, C2 (unit-cell parameters a = 124.7, b = 51.4, c = 103.6 A, beta = 122.8) and R3 (a = b = 122.6, c = 97.6 A)
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