6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Reaction

Synonyms

Asuc_1947, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -glutamate-cysteine ligase-glutathione synthetase, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GCL, GCSGS, ghF, glutamate cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, Glutathione synthase, Glutathione synthetase, Glutathione synthetase (tripeptide), GS, GSH synthase, GSH synthetase, GSH-S, GSH2, gshAB, GSHase, gshB, GshF, GshFAp, GshFAs, GSHII, GSHS, GSHS1, GSS, hGS, L-glutathione synthetase, More, Phytochelatin synthetase, StGCL-GS, Synthetase, glutathione, TAGS1, TaGS2, TbGS, ZmGS

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.3 glutathione synthase

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Results	in table
15	Activating Compound
21524	AA Sequence
8	Application
1	CAS Registry Number
64	Cloned(Commentary)
43	Cofactor
13	Crystallization (Commentary)
527	Disease/ Diagnostics
2	Expression
28	General Information
6	General Stability
1	IC50 Value
82	Inhibitors
9	kcat/KM [mM/s]
19	Ki Value [mM]
216	KM Value [mM]
19	Localization
62	Metals/Ions
40	Molecular Weight [Da]
62	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
1904	Organism
7	Pathway
29	PDB ID
35	pH Optimum
1	pH Range
2	pH Stability
4	pI Value
7	Posttranslational Modification
99	Protein Variants
32	Purification (Commentary)
9	Reaction
2	Reaction Type
141	Reference
74	Source Tissue
39	Specific Activity [micromol/min/mg]
10	Storage Stability
241	Substrates and Products (Substrate)
36	Subunits
139	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
1	Temperature Range [°C]
20	Temperature Stability [°C]
103	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.3.2.3 - glutathione synthase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-18°C, stable for at least 1 year

[Candida] boidinii

-

1188

-20°C, 20% loss of activity after 2 months

Escherichia coli

-

1196

-20°C, purified enzyme, 30% glycerol, at least 2 years stable

Rattus norvegicus

-

650833

-20°C, stable for weeks

Escherichia coli

-

1192

-80°C, 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, can be stored for up to 6 months without significant loss of activity

Pasteurella multocida

-

662457

-80°C, in 50 mM Tris-HCl (pH 7.4) containing100 mM NaCl, up to 6 months, without significant loss of activity

Pasteurella multocida

-

662457

4°C or frozen, in presence of glutathione and 5 mM DTT, stable for 1 month

Bos taurus

-

1198

4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol

Streptococcus agalactiae

-

662339

4°C, when stored in 20 mM Tris-Cl and 1 mM EDTA, pH 8.6, in sterile cryogenic tubes at 4°C, the recombinant hGS dimer interface mutant enzymes lose activity in the 30 hours after purification. Both R221A and D24A lose activity in a biphasic manner within a few hours: D24A loses 30% of activity in 4 hours, while the activity of R221A decreases by 20% in 7.5 hours

Homo sapiens

P48637

746387

stable for more than 2 months

Rattus norvegicus

-

1194