6.3.2.25: tubulin-tyrosine ligase

This is an abbreviated version!
For detailed information about tubulin-tyrosine ligase, go to the full flat file.

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Synonyms

Synthetase, tubulin-tyrosine, TTL, TTLase, tubulin tyrosine ligase, tubulin-tyrosine ligase, tubulin-tyrosine-ligase, Tubulin:tyrosine ligase, tubuline-tyrosine ligase, Tyrosyltubulin ligase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.25 tubulin-tyrosine ligase

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Results	in table
2	Activating Compound
178	AA Sequence
2	Application
1	CAS Registry Number
8	Cloned(Commentary)
5	Cofactor
1	Crystallization (Commentary)
174	Disease/ Diagnostics
9	General Information
2	General Stability
30	Inhibitors
1	Ki Value [mM]
14	KM Value [mM]
4	Localization
16	Metals/Ions
12	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
40	Organism
5	PDB ID
1	pH Optimum
1	pI Value
3	Posttranslational Modification
9	Protein Variants
8	Purification (Commentary)
3	Reaction
1	Reaction Type
40	Reference
43	Source Tissue
5	Specific Activity [micromol/min/mg]
5	Storage Stability
46	Substrates and Products (Substrate)
7	Subunits
36	Synonyms
1	Systematic Name

Substrates Products

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Substrates Products on EC 6.3.2.25 - tubulin-tyrosine ligase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

alpha-tubulin + ADP + phosphate

ATP + detyrosinated alpha-tubulin + L-tyrosine

Bos taurus

-

-

1175

-

-

r

ATP + detyrosinated alpha-tubulin + 3-fluoro-Tyr

?

Gallus gallus

-

tubulin covalently labeled with 3-fluoro-Tyr is competient to polimerize into microtubules

1179

-

-

?

ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine

nitrotyrosinated alpha-tubulin + ADP + phosphate

3 entries

ATP + detyrosinated alpha-tubulin + iodotyrosine

?

Sus scrofa

-

-

1168

-

-

?

ATP + detyrosinated alpha-tubulin + L-DOPA

?

Sus scrofa

-

-

1166, 1184

-

-

?

ATP + detyrosinated alpha-tubulin + L-hydroxyphenylalanine

?

Bos taurus

-

-

1175

-

-

?

ATP + detyrosinated alpha-tubulin + L-Phe

?

2 entries

ATP + detyrosinated alpha-tubulin + L-Tyr

?

5 entries

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

7 entries

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

13 entries

ATP + detyrosinated alpha-tubulin + tyramine

?

2 entries

ATP + peptide of detyrosinated alpha tubulin + Tyr

peptide of alpha-tubulin + ADP + phosphate

Sus scrofa

-

peptides ending like alpha-tubulin with the sequence Gly-Glu-Glu are optimally tyrosinated once a peptide length of 12 residues is reached. The carboxy-terminal tetradecapeptide of detyrosinated alpha-tubulin acts as substrate at 50fold lower efficiency than alphabeta-tubulin

1167

-

-

?

additional information

?

-

4 entries

ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine

nitrotyrosinated alpha-tubulin + ADP + phosphate

Nicotiana tabacum

-

-

709317

-

-

ir

ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine

nitrotyrosinated alpha-tubulin + ADP + phosphate

Oryza sativa Japonica Group

-

-

709317

-

-

ir

ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine

nitrotyrosinated alpha-tubulin + ADP + phosphate

Sus scrofa

-

35fold lower affinity for nitrotyrosine than for L-tyrosine, nitrotyrosine is incoorporated into the C-terminus

650316

-

650316

?

ATP + detyrosinated alpha-tubulin + L-Phe

?

Bos taurus

-

-

1175

-

-

?

ATP + detyrosinated alpha-tubulin + L-Phe

?

Sus scrofa

-

-

1166, 1184

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

?

Bos taurus

-

posttranslational addition of Tyr to the carboxyterminal end of detyrosinated alpha-tubulin

1172

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

?

Bos taurus

-

posttranslational modification of tubulin with potential significance for the regulation of microtubule assembly or function

1175

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

?

Caenorhabditis elegans

-

activity increases from the embryonic stage to the fourth larval stage L4, decreases from L4 to adult and to aged nematodes with only qualitative alterations in substrate specificity

1176

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

?

Crithidia fasciculata

-

terminal Tyr is present in about 5% of flagellar alpha-chain from cells in stationary phase and 20% from cells from midlog phase. None is detected in tubulin from cytosol or the subpellicular corset. In contrast to mammalian cells, in which higher state of tyrosinylation characterizes recently assembled or unstable microtubules, terminal Tyr is present only in the most stable polymer, the flagellar double microtubules

1169

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

?

Sus scrofa

-

posttranslational addition of Tyr to the carboxyterminal end of detyrosinated alpha-tubulin

1172

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Crithidia fasciculata

-

tyrosinates Crithidia tubulin but not brain tubulin and is specific for the alpha chain

1169

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Homo sapiens

Q8NG68

the enzyme plays a vital role in neuronal organization

663250

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Mus musculus

-

the enzyme plays a vital role in neuronal organization

663250

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Rattus norvegicus

-

r

1174

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

1166

-

1166

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

1167, 1168

-

-

?

ATP + detyrosinated alpha-tubulin + L-Tyr

alpha-tubulin + ADP + phosphate

Sus scrofa

-

turkey erythrocyte tubulin

1177

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Bos taurus

-

-

728153

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Bos taurus

-

-

1178

-

-

r

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Gallus gallus

E1BQ43

-

728025

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Gallus gallus

E1BQ43

tubuline alpha/beta-dimers, no activity with tyrosinate polymerized microtubules in which the tubulin subunits adopt a straighter configuration

728025

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Homo sapiens

-

-

652600

-

652600

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Mus musculus

-

-

686295, 728025

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Mus musculus

-

tubulin tyrosination is a major factor affecting the recruitment of cytoskeleton-associated protein glycine-rich proteins at microtubule plus ends

675003

-

-

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Rattus norvegicus

Q9QXJ0

-

653353

-

653353

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

650316

-

650316

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

650790

-

650790

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

652600

-

652600

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

653350

-

653350

?

ATP + detyrosinated alpha-tubulin + L-tyrosine

alpha-tubulin + ADP + phosphate

Sus scrofa

-

-

728153

-

-

?

ATP + detyrosinated alpha-tubulin + tyramine

?

Bos taurus

-

-

1175

-

-

?

ATP + detyrosinated alpha-tubulin + tyramine

?

Sus scrofa

-

very low activity

1184

-

-

?

additional information

?

-

Bos taurus

-

full-length stathmin and tubulin tyrosine ligase compete for binding to tubulin and fail to make a stable tubulin:stathmin:tubulin tyrosine ligase triple complex in solution

728153

-

-

?

additional information

?

-

Homo sapiens

-

the testis-specific apoptosis related gene TTL.6 (member of the tubulin-tyrosine ligase family) undergoes adaptive evolution in the lineage leading to humans

673831

-

-

?

additional information

?

-

Mus musculus

-

microtubule-associated protein 1B interaction with tubulin tyrosine ligase contributes to the control of microtubule tyrosination, interaction between MAP1B and TTL is not regulated by MAP1B phosphorylation, overview

686295

-

-

?

additional information

?

-

Sus scrofa

-

full-length stathmin and tubulin tyrosine ligase compete for binding to tubulin and fail to make a stable tubulin:stathmin:tubulin tyrosine ligase triple complex in solution

728153

-

-

?