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6.3.2.2: glutamate-cysteine ligase

This is an abbreviated version!
For detailed information about glutamate-cysteine ligase, go to the full flat file.

Word Map on EC 6.3.2.2

Reaction

ATP
+
L-glutamate
+
L-cysteine
=
ADP
+
phosphate
+
gamma-L-glutamyl-L-cysteine

Synonyms

Ace-GCL, Asuc_1947, AtGCL, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -GCS, gamma-ECL, Gamma-ECS, gamma-GC, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase-glutathione synthetase, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutaminylcysteine synthetase, gamma-glutamycysteine synthetase, gamma-glutamyl-cysteine ligase, gamma-Glutamyl-L-cysteine synthetase, gamma-glutamylcysteine ligase, gamma-glutamylcysteine synthase, gamma-Glutamylcysteine synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, gamma-Glutamylcysteinyl-synthetase, gammaGCS, GCL, GCLC, Gclc-X2, GCLM, GCS, GCSGS, ghF, GLCL, GLCLC, GLCLR, glutamate cysteine ligase, glutamate cysteine ligase gene, glutamate-cysteine ligase, glutamate-cysteine-ligase, glutamate–cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, GSH1, GshA, gshAB, GshF, GshFAp, GshFAs, GSHI, I79_022778, L-glutamate L-cysteine ligase, More, PAD2, PhGshA II, PSHAa0937, StGCL-GS, Synthetase, gamma-glutamylcysteine

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.2 glutamate-cysteine ligase

Specific Activity

Specific Activity on EC 6.3.2.2 - glutamate-cysteine ligase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.001
native COS cells
0.0027
-
lung extract exposed to air
0.0041
-
lung extract exposed to cadmium aerosol with 3.3 mg Cd/m3
0.0048
-
lung extract exposed to cadmium aerosol with 4.2 mg Cd/m3
0.0097
-
partially purified enzyme from astrocytes
0.014
transformed COS cells expressing both the recombinant subunits at equal amounts
0.031
-
recombinant wild-type enzyme
0.038
transformed COS cells expressing the recombinant catalytic subunit
0.05
-
recombinant mutant S495T
0.051
-
recombinant mutant A494V and mutant A494L
0.12
-
purified recombinant mutant C249G catalytic subunit and mutant C295G catalytic subunit
0.17
-
purified recombinant mutant C52G catalytic subunit and mutant C248G catalytic subunit
0.38
-
purified recombinant mutant C605G catalytic subunit
0.44
-
purified mutant R127C enzyme, substrates L-glutamate and L-alpha-aminobutyrate
0.48
-
purified recombinant mutant C501G catalytic subunit
0.63
-
purified recombinant mutant C491G catalytic subunit
0.92
-
purified recombinant mutant C553G catalytic subunit
1.83
-
purified recombinant mutant C553G holoenzyme
14.5
-
liver enzyme
19.2
-
kidney enzyme
28.75
-
-
39.5
-
pH 8.2
4.67
-
purified recombinant mutant C249G holoenzyme
5.17
-
purified recombinant mutant C605G holoenzyme
5.33
-
purified recombinant mutant C295G holoenzyme
5.67
-
purified recombinant mutant C248G holoenzyme
51
purified enzyme
6.67
-
purified recombinant mutant C52G holoenzyme
7
-
purified recombinant mutants C491G holoenzyme and C501G holoenzyme
8.39
-
Drosophila catalytic subunit as a hybrid with the wild-type human modifier subunit, substrate L-alpha-aminobutyrate
8.45
-
wild-type holoenzyme
8.86
-
purified wild-type enzyme, substrates L-glutamate and L-alpha-aminobutyrate
additional information