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0.002 - 0.0029
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
0.00193
(6R)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00087
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.00096
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0016
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.00105
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0145
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
1
(6RS)-5,6,7,8-tetrahydrofolic acid
-
pH 9.75
0.00106
(6S)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00116
(6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.0014 - 0.0063
(6S)-5,6,7,8-tetrahydropteroylglutamate
0.075 - 0.122
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
0.054 - 0.064
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
0.0022 - 0.014
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.41
3,3-difluoroglutamate
-
reaction with methotrexate + ATP
0.0031 - 0.0054
3,3-difluoromethotrexate
0.0012 - 0.0076
5,10-dideazatetrahydrofolic acid
0.03 - 3.8
5,10-methylene-5,6,7,8-tetrahydrofolic acid
0.01 - 0.204
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
0.0048 - 0.057
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
0.0023
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.00096
5,6,7,8-tetrahydrofolate
-
-
0.00123
5,6,7,8-tetrahydrofolate-Glu2
-
pH 8.7
0.01 - 0.655
5,6,7,8-tetrahydrofolic acid
0.16
5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu
-
L-Glu
-
0.3
5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)2
mutant D154A, pH 9.8
0.074
5,6,7,8-tetrahydropteroyl-(4-aminobutanoyl)-Glu
-
-
0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-Glu
-
L-Glu
0.018 - 0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
0.0021 - 6.8
5,6,7,8-tetrahydropteroyl-Glu
0.0033 - 0.0034
5,6,7,8-tetrahydropteroyl-Glu2
0.0011 - 0.0014
5,6,7,8-tetrahydropteroyl-Glu3
0.0016 - 0.002
5,6,7,8-tetrahydropteroyl-Glu4
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
0.0081 - 0.105
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.013
5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.444
5-methyl-H4-homofolate
-
-
0.0063 - 0.98
7,8-dihydropteroate
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
0.022
7,8-dihydropteroyl-(5-aminopentanoyl)-Glu
-
-
0.076
7,8-dihydropteroyl-beta-Ala-Glu
-
-
0.00081 - 0.0086
7,8-dihydropteroyl-Glu
0.0026 - 0.048
7,8-dihydropteroyl-Glu2
0.05
dATP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.0073
dihydroaminopterin
-
-
0.235
glutamic acid
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.136
ITP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.43 - 350
L-Glutamic acid
0.019 - 0.138
methotrexate
0.063 - 0.074
methotrexate-Glu
0.029
methotrexate-Glu2
-
-
0.011
methotrexate-Glu4
-
methotrexate-Glu5
0.126 - 0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
0.81
pteroyl-(4-aminobutanoyl)-Glu
-
-
1.09
pteroyl-(5-aminopentanoyl)-Glu
-
-
0.69
pteroyl-beta-Ala-Glu
-
-
0.059 - 0.137
pteroyl-Glu
0.962
pteroylglutamate
-
-
0.093
pteroylmonoglutamate
-
-
0.062
pteroylmonoglutamyl-Glu
0.19
UTP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.002
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.0029
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.0014
(6S)-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.0063
(6S)-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.075
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.122
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.054
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.064
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.0022
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0037
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0039
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.014
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0031
3,3-difluoromethotrexate
-
-
0.0054
3,3-difluoromethotrexate
-
-
0.0012
5,10-dideazatetrahydrofolic acid
-
pH 8.9, 37ºC, L1210 isoform
0.0076
5,10-dideazatetrahydrofolic acid
-
pH 8.9, 37ºC, liver isoform
0.03
5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
pH 9.75
0.032
5,10-methylene-5,6,7,8-tetrahydrofolic acid
wild-type
0.034
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant S152A, pH 9.8
0.048
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant Y414A, pH 9.8
0.05
5,10-methylene-5,6,7,8-tetrahydrofolic acid
wild-type
0.071
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.071
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.084
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant R15E, pH 9.8
0.11
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant D151A, pH 9.8
0.116
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant S152W, pH 9.8
0.118
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-71 of Lactobacillus casei enzyme, residues 82-93 of Escherichia coli enzyme, and residues 83-428 of Lactobacillus casei enzyme, pH 9.8
0.118
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-71 of Lactobacillus casei enzyme, residues 82-93 of Escherichia coli enzyme, and residues 83-428 of Lactobacillus casei enzyme, pH 9.8
0.23
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant F75A, pH 9.8
0.59
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.59
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
1.5
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant F121A, pH 9.8
3.8
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant T119W, pH 9.8
0.01
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
mutant D151A, pH 9.8
0.204
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
mutant R15E, pH 9.8
0.0048
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.027
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.057
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.01
5,6,7,8-tetrahydrofolic acid
mutant A155W, pH 9.8
0.0315
5,6,7,8-tetrahydrofolic acid
pH 7.5, wild type enzyme
0.071
5,6,7,8-tetrahydrofolic acid
pH 7.5, E81A mutant
0.094
5,6,7,8-tetrahydrofolic acid
pH 7.5, S412A mutant
0.21
5,6,7,8-tetrahydrofolic acid
pH 7.5, R82A mutant
0.24
5,6,7,8-tetrahydrofolic acid
pH 7.5, K185A mutant
0.34
5,6,7,8-tetrahydrofolic acid
mutant D154A, pH 9.8
0.39
5,6,7,8-tetrahydrofolic acid
mutant T122W, pH 9.8
0.4
5,6,7,8-tetrahydrofolic acid
mutant T122H, pH 9.8
0.448
5,6,7,8-tetrahydrofolic acid
mutant A155H, pH 9.8
0.655
5,6,7,8-tetrahydrofolic acid
pH 7.5, S73A mutant
0.018
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
-
-
0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
L-Glu
-
0.0021
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0035
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0044
5,6,7,8-tetrahydropteroyl-Glu
-
aminopterin
0.006
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.007
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0077
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.009
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.163
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.336
5,6,7,8-tetrahydropteroyl-Glu
-
-
6.8
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0033
5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.0034
5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.0011
5,6,7,8-tetrahydropteroyl-Glu3
-
5,6,7,8-tetrahydropteroyl-Glu5
0.0014
5,6,7,8-tetrahydropteroyl-Glu3
-
-
0.0016
5,6,7,8-tetrahydropteroyl-Glu4
-
-
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
-
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
7,8-dihydropteroyl-Glu
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
5,6,7,8-tetrahydropteroyl-Glu
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
-
-
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
0.0081
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.105
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0063
7,8-dihydropteroate
wild-type, pH 9.8
0.0063
7,8-dihydropteroate
wild-type, pH 9.8
0.08
7,8-dihydropteroate
mutant A155W, pH 9.8
0.134
7,8-dihydropteroate
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.134
7,8-dihydropteroate
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.14
7,8-dihydropteroate
mutant D154A, pH 9.8
0.15
7,8-dihydropteroate
mutant T122W, pH 9.8
0.485
7,8-dihydropteroate
mutant A155H, pH 9.8
0.98
7,8-dihydropteroate
mutant T122H, pH 9.8
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
-
-
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
-
pteroyl-Glu4
0.00081
7,8-dihydropteroyl-Glu
-
-
0.0009
7,8-dihydropteroyl-Glu
-
-
0.005
7,8-dihydropteroyl-Glu
-
-
0.0086
7,8-dihydropteroyl-Glu
-
-
0.0026
7,8-dihydropteroyl-Glu2
-
-
0.048
7,8-dihydropteroyl-Glu2
-
-
0.004
aminopterin
-
-
0.0059
aminopterin
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.0065
aminopterin
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
0.009
aminopterin
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.0104
aminopterin
-
pH 8.7
0.011
aminopterin
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.013
aminopterin
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.0154
aminopterin
-
pH 8.9, 37ºC, L1210 isoform
0.017
aminopterin
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
0.0177
aminopterin
-
pH 8.9, 37ºC, liver isoform
0.025
aminopterin
-
pteroyl-3,3-difluoroglutamate
0.031
aminopterin
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
0.18
aminopterin
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
0.0046
ATP
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.01
ATP
-
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.01
ATP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.016
ATP
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
0.028
ATP
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
0.037
ATP
mutant D154A, pH 9.8 cosubstrate 7,8-dihydropteroate
0.04
ATP
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.045
ATP
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.046
ATP
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.054
ATP
wild-type, pH 9.8
0.06
ATP
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
0.065
ATP
mutant A155H, pH 9.8 cosubstrate 7,8-dihydropteroate
0.0665
ATP
-
reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
0.07
ATP
-
pH 9.75, temperature conditions not mentioned
0.076
ATP
mutant A155H, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.18
ATP
mutant F75A, pH 9.8
0.23
ATP
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.23
ATP
mutant Y414A, pH 9.8
0.25
ATP
-
reaction with aminopterin + ATP
0.267
ATP
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
0.555
ATP
mutant A155W, pH 9.8 cosubstrate 7,8-dihydropteroate
0.62
ATP
pH 7.5, S412A mutant
0.87
ATP
pH 7.5, R82A mutant
2.5
ATP
pH 7.5, S73A mutant
2.9
ATP
pH 7.5, K185A mutant
3.4
ATP
pH 7.5, wild type enzyme
3.4
ATP
mutant D151A, pH 9.8
3.4
ATP
wild-type, pH 9.8
5.2
ATP
pH 7.5, E81A mutant
5.7
ATP
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
5.7
ATP
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.332
Glu
-
-
0.333
Glu
-
reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.346
Glu
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.74
Glu
-
reaction with methotrexate + ATP
1.63
Glu
-
mutant enzyme A447V
1.702
Glu
-
wild type enzyme from cytosol
2.068
Glu
-
wild type enzyme from mitochondrion
2.281
Glu
-
mutant enzyme S457F
5.984
Glu
-
mutant enzyme R424C
0.053
L-Glu
mutant F75A, pH 9.8
0.12
L-Glu
mutant A155H, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.17
L-Glu
mutant A155H, pH 9.8, cosubstrate 7,8-dihydropteroate
0.2
L-Glu
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.292
L-Glu
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.292
L-Glu
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.3
L-Glu
wild-type, pH 9.8
0.47
L-Glu
wild-type, pH 9.8
1.2
L-Glu
-
reaction with aminopterin + ATP
2.7
L-Glu
mutant Y414A, pH 9.8
3.3
L-Glu
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.24
L-glutamate
-
pH 8.7
0.41
L-glutamate
pH 7.5, E81A mutant
0.47
L-glutamate
pH 7.5, wild type enzyme
0.85
L-glutamate
pH 7.5, S73A mutant
1
L-glutamate
-
pH 8.5, 37ºC, wild type cell line
2 - 3
L-glutamate
-
pH 8.5, 37ºC, MTXR5 cell subline
2.06
L-glutamate
pH 7.5, R82A mutant
5.06
L-glutamate
pH 7.5, K185A mutant
26.69
L-glutamate
pH 7.5, S412A mutant
0.43
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.52
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.83
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
2.9
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
91
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
140
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
350
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.019
methotrexate
-
-
0.04
methotrexate
-
pH 8.7
0.0526
methotrexate
-
wild type enzyme from mitochondrion
0.054
methotrexate
-
5-methyl-5,6,7,8-tetrahydrpteroyl-Glu
0.0555
methotrexate
-
wild type enzyme from cytosol
0.0613
methotrexate
-
mutant enzyme S457F
0.0827
methotrexate
-
mutant enzyme A447V
0.0908
methotrexate
-
mutant enzyme R424C
0.1
methotrexate
-
aminopterin
0.063
methotrexate-Glu
-
pH 8.5, 37ºC, wild type cell line
0.074
methotrexate-Glu
-
pH 8.5, 37ºC, MTXR5 cell subline
0.02
methotrexate-Glu3
-
-
0.02
methotrexate-Glu3
-
pteroyl-Glu3
0.126
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
pH 8.7
0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
-
0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
-
0.059
pteroyl-Glu
-
-
0.062
pteroylmonoglutamyl-Glu
-
-
0.062
pteroylmonoglutamyl-Glu
-
pteroyl-Glu2