6.3.2.13: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase, go to the full flat file.

Reaction

Synonyms

amide ligase, Diaminopimelic-adding enzyme, mDAP ligase, Meso-diaminopimelate-adding enzyme, Mur ligase, MurE, MurE synthetase, MurEEr, peptidoglycan MurE ligase, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyl-meso-2,6-diaminopimelate, UDP-MurNAc-tripeptide synthetase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelate synthetase, UDP-N-acetylmuramyl tripeptide synthetase, UDP-N-acetylmuramyl-tripeptide synthetase, UDPMurNAc-L-alanyl-D-glutamate:mDAP ligase (ADP-forming), Uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase, Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.13 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

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Results	in table
32413	AA Sequence
1	CAS Registry Number
11	Cloned(Commentary)
3	Cofactor
4	Crystallization (Commentary)
7	General Information
13	IC50 Value
27	Inhibitors
8	kcat/KM [mM/s]
57	KM Value [mM]
13	Localization
23	Metals/Ions
4	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
38	Organism
7	Pathway
42	PDB ID
9	pH Optimum
2	pH Range
16	Protein Variants
11	Purification (Commentary)
3	Reaction
3	Reaction Type
31	Reference
6	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
82	Substrates and Products (Substrate)
2	Subunits
35	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
15	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 6.3.2.13 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu-meso-2,6-diaminoheptanedioate

Escherichia coli

-

-

1027

-

1027

?

ATP + MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Mycobacterium tuberculosis

-

relative activity wild-type: 14%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + D-Ala-D-Ala

?

Mycobacterium tuberculosis

-

relative activity wild-type: 5%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + D-allo-cystathionine

ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-D-allo-cystathionine

Escherichia coli

-

-

1038

-

1038

?

ATP + UDP-MurNAc-L-Ala-D-Glu + D-Glu

?

Mycobacterium tuberculosis

-

relative activity wild-type: 13%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + D-Lys

?

Mycobacterium tuberculosis

-

relative activity wild-type: 9%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + D-ornithine

?

Mycobacterium tuberculosis

-

relative activity wild-type: 8%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + DL-lanthionine

?

Mycobacterium tuberculosis

-

relative activity wild-type: 82%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + L-Ala

?

Mycobacterium tuberculosis

-

relative activity wild-type: 12%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + L-allo-cystathionine

ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-L-allo-cystathionine

Escherichia coli

-

-

1038

-

1038

?

ATP + UDP-MurNAc-L-Ala-D-Glu + L-cystathionine

?

Mycobacterium tuberculosis

-

relative activity wild-type: 12%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + L-Lys

?

Mycobacterium tuberculosis

-

relative activity wild-type: 7%

716815

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

10 entries

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-lanthionine

ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-meso-lanthionine

Escherichia coli

-

-

1038

-

1038

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate + L-alanine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycyl-gamma-D-glutamyl-L-alanine

Erysipelothrix rhusiopathiae

E7FUC9

-

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + (2S,6S)-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-(2S,6S)-2,6-diaminoheptanedioate

Erysipelothrix rhusiopathiae

E7FUC9

7.8% activity with (2S,6S)-2,6-diaminoheptanedioate compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + beta-chloro-L-alanine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-beta-chloro-L-alanine

Erysipelothrix rhusiopathiae

E7FUC9

15% activity with beta-chloro-L-alanine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + glycine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-glycine

Erysipelothrix rhusiopathiae

E7FUC9

7% activity with glycine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-alanine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-alanine

Erysipelothrix rhusiopathiae

E7FUC9

highest activity with L-alanine compared to the other amino acids

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-diaminobutyrate

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-diaminobutyrate

Erysipelothrix rhusiopathiae

E7FUC9

7.9% activity with L-diaminobutyrate compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-homoserine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-homoserine

Erysipelothrix rhusiopathiae

E7FUC9

10% activity with L-homoserine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-propargylglycine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-propargylglycine

Erysipelothrix rhusiopathiae

E7FUC9

20% activity with L-propargylglycine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-serine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-serine

Erysipelothrix rhusiopathiae

E7FUC9

30% activity with L-serine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-vinylglycine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-vinylglycine

Erysipelothrix rhusiopathiae

E7FUC9

58% activity with L-vinylglycine compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate

2 entries

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate + L-alanine

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-gamma-D-glutamyl-L-alanine

Erysipelothrix rhusiopathiae

E7FUC9

-

744440

-

-

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid

2 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine

2 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine

3 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine

2 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

7 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

3 entries

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

2 entries

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

5 entries

GTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

GDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium tuberculosis

-

relative activity wild-type: 30%

716815

-

-

?

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

2 entries

UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

3 entries

additional information

?

-

6 entries

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus cereus

-

r

1030, 1031

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus cereus

-

specific for UDP-N-MurNAc-L-Ala-D-Glu and meso-2,6-diaminoheptanedioate

1030

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus subtilis

-

-

1294

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus subtilis 168

-

-

1294

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Escherichia coli

-

-

1027, 1034, 1035, 1036, 1037, 1038, 1276

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Escherichia coli

-

r

1039

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Lysinibacillus sphaericus

-

-

985, 1033

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Lysinibacillus sphaericus 9602

-

-

985, 1033

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium leprae

O69557

the threedimensional structure of MurE from Mycobacterium leprae is modeled using comparative modeling methods based on the X-ray crystal structure of MurE from Escherichia coli. The docked complexes reveal the amino acids responsible for binding the substrates. Superposition of these complex structures suggests that carboxylic acid group of UDP-Nacetyl muramoyl-glycyl-D-glutamate is positioned in proximity to gamma-phosphate of the ATP to facilitate the formation of acylphosphate intermediate. The orientation of an amino group of meso-diaminopimelic acid facilitates the nucleophilic attack to form the product

715975

-

-

?

ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium tuberculosis

-

relative activity wild-type: 100%

716815

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate

Erysipelothrix rhusiopathiae

E7FUC9

-

744440

-

-

?

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate

Erysipelothrix rhusiopathiae

E7FUC9

30% activity with meso-2,6-diaminoheptanedioate compared to L-alanine

744440

-

-

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid

Mycobacterium tuberculosis

P9WJL3

-

716979

-

-

?

ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid

Mycobacterium tuberculosis H37Rv

P9WJL3

-

716979

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine

Thermotoga maritima

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine

Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine

Staphylococcus aureus

-

-

677175

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine

Thermotoga maritima

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine

Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine

Thermotoga maritima

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine

Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Escherichia coli

-

-

651110

-

651110

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Escherichia coli

-

-

674330

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Escherichia coli

P22188

-

652097

-

652097

r

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Escherichia coli

P22188

part of bacterial cell-wall peptidoglycan biosynthesis

652097

-

652097

r

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Pseudomonas aeruginosa

-

-

645342

-

645342

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Staphylococcus aureus

-

-

653141

-

653141

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate

Staphylococcus aureus

-

essential for optimal expression of methicillin resistance

653141

-

653141

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium leprae

O69557

-

715975

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium tuberculosis

P9WJL3

-

728598

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium tuberculosis H37Rv

P9WJL3

-

728598

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

Thermotoga maritima

-

-

674565

-

-

?

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589

-

-

674565

-

-

?

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Escherichia coli

-

catalyzes an early reaction in the biosynthesis of bacterial peptidoglycan

1036

-

-

?

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Escherichia coli

-

the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis

1276

-

-

?

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Escherichia coli

-

enzyme is not essential for growth

1034

-

-

?

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Lysinibacillus sphaericus

-

enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation

1033

-

-

?

ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

?

Lysinibacillus sphaericus 9602

-

enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation

1033

-

-

?

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

Mycobacterium tuberculosis

-

-

715309

-

-

?

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid

ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid

Pseudomonas aeruginosa

-

assay at pH 8.0

702135

-

-

?

UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus cereus

-

-

1031

-

-

?

UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Bacillus cereus

-

16% of the activity relative to ATP

1030

-

1030

?

UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate

UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

Mycobacterium tuberculosis

-

relative activity wild-type: 17%

716815

-

-

?

additional information

?

-

Erysipelothrix rhusiopathiae

E7FUC9

enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview

744440

-

-

?

additional information

?

-

Erysipelothrix rhusiopathiae

-

enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview

744440

-

-

?

additional information

?

-

Mycobacterium tuberculosis

P9WJL3

highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity

716979

-

-

?

additional information

?

-

Mycobacterium tuberculosis

P9WJL3

MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP

728598

-

-

?

additional information

?

-

Mycobacterium tuberculosis H37Rv

P9WJL3

highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity

716979

-

-

?

additional information

?

-

Mycobacterium tuberculosis H37Rv

P9WJL3

MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP

728598

-

-

?