6.3.2.10: UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase, go to the full flat file.
Word Map on EC 6.3.2.10
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6.3.2.10
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atrophy
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atrogin-1
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ligases
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wasting
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muscle-specific
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myotubes
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proteasome
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fiber
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mafbx
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gastrocnemius
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ubiquitin-proteasome
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hypertrophy
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myostatin
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soleus
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disuse
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cachexia
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denervation
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myosin
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sarcopenia
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atrogenes
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myogenin
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forkhead
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myod
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atrophy-related
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tibialis
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foxo3a
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hindlimb
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igf-1
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myofiber
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unloading
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cachectic
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vastus
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denervation-induced
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finger-1
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dexamethasone-induced
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lateralis
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myofibrillar
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ring-finger
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medicine
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drug development
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gabarapl1
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e3-ligases
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autophagy-lysosome
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pharmacology
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immobilization-induced
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e3-ubiquitin
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murfs
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sarcomeric
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slow-twitch
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muscle-wasting
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3-methylhistidine
- 6.3.2.10
- atrophy
-
atrogin-1
- ligases
- wasting
-
muscle-specific
- myotubes
- proteasome
- fiber
-
mafbx
-
gastrocnemius
-
ubiquitin-proteasome
- hypertrophy
-
myostatin
- soleus
-
disuse
-
cachexia
-
denervation
- myosin
- sarcopenia
-
atrogenes
-
myogenin
-
forkhead
- myod
-
atrophy-related
- tibialis
-
foxo3a
- hindlimb
- igf-1
-
myofiber
-
unloading
-
cachectic
-
vastus
-
denervation-induced
-
finger-1
-
dexamethasone-induced
- lateralis
-
myofibrillar
-
ring-finger
- medicine
- drug development
-
gabarapl1
-
e3-ligases
-
autophagy-lysosome
- pharmacology
-
immobilization-induced
-
e3-ubiquitin
-
murfs
-
sarcomeric
-
slow-twitch
-
muscle-wasting
- 3-methylhistidine
Reaction
Synonyms
AbMurF, ATP-dependent UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase, D-Ala-D-Ala-adding enzyme, More, MurF, MurF1, Synthetase, uridine diphosphoacetylmuramoylpentapeptide, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase, UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase, UDPacetylmuramoylpentapeptide synthetase, Uridine diphosphoacetylmuramoylpentapeptide synthetase
ECTree
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Crystallization
Crystallization on EC 6.3.2.10 - UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
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crystallization of two MurF-ATP complexes: the MurFATP complex and the MurF-ATP-UDP complex, trigonal crystals of Se-AbMurF with ATP are grown in a precipitant solution containing 20% w/v PEG 3000 and 0.1 M sodium citrate-citric acid, pH 5.5, crystals of the AbMurF-ATP-UDP complex are obtained by soaking experiments, trigonal crystals of the AbMurF-ATP complex isomorphous to those of the Se-AbMurF-ATP complex are grown in the mother liquor and are then transferred to a solution containing 20% glycerol, 20% PEG 3000, 0.1 M sodium citrate-citric acid, pH 5.5, and 15 mM UDP, 25 min soaking, monoclinic crystals of the AbMurF-ATP complex are grown in a precipitant solution containing 24% PEG 4000, 0.08 M Tris-HCl, pH 8.5, 0.16 M MgCl2, and 20% glycerol by the micro-batch crystallization method at 22°C, X-ray diffraction structure determination and analysis at 1.8-2.8 A resolution
purified MurF in complex with ATP, microbatch crystallization method, mixing of 0.002 ml of 15 mg/ml protein in 150 mM NaCl, 20 mM HEPES, pH 7.4, and 1 mM DTT, with 0.002 ml of reservoir solution containing 0.1 M sodium citratecitric acid pH 5.5, 20% PEG 3000, 22°C, X-ray diffraction structure determination and analysis at 1.90 A resolution
hanging-drop vapour diffusion method, purified recombinant enzyme 12.2 mg/ml, in 10 mM Tris-HCl, pH 7.4, 10 mM DTT, plus equal volume of reservoir solution: 0.1 M Bis-Tris propane buffer, pH 9.4, 18% PEG 8000, 0.12 M MgSO4, 10% glycerol, about 20 days, X-ray diffraction structure determination at 2.8 A resolution, and analysis