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6.3.2.1: pantoate-beta-alanine ligase (AMP-forming)

This is an abbreviated version!
For detailed information about pantoate-beta-alanine ligase (AMP-forming), go to the full flat file.

Word Map on EC 6.3.2.1

Reaction

ATP
+
(R)-pantoate
 +
beta-Alanine
=
AMP
 +
diphosphate
 +
(R)-pantothenate

Synonyms

D-Pantoate:beta-alanine ligase (AMP-forming), MTBPS, PanC, panthotenate synthetase, Pantoate activating enzyme, pantoate-beta-alanine ligase, Pantoic-activating enzyme, pantothenate, Pantothenate synthetase, PS, PTS, Rv3602c, synthetase, Synthetase, pantothenate

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.1 pantoate-beta-alanine ligase (AMP-forming)

Cloned

Cloned on EC 6.3.2.1 - pantoate-beta-alanine ligase (AMP-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
expression Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as N-terminal His-tagged protein
-
expression in Escherichia coli BL21 as N-terminal His-tagged protein
-
gene panC, expression in Escherichia coli strain BL21(DE3)
gene panC, expression of His6-tagged maltose-binding protein fusion enzyme in Escherichia coli strain BL21(DE3)
gene panC, recombinant expression as His-tagged enzyme, and expression in and complementation of the Arabidopsis thaliana knockout mutant phenotype by heterologous expression of Escherichia coli PTS, the panC transgene increases the total PTS activity in leaves of trangenic plants by up to 500fold but does not affect the steady-state level of pantothenate, overview
-
gene panC, recombinant expression in Escherichia coli strain BL21(DE3)
gene panC, recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3)
overexpression as a fusion protein with cytochrome b5 in Escherichia coli BL21(DE3). The advantages of the cytochrome b5 fusion system are its high expression levels in both rich and minimal medium, high solubility, stability, ease of purification, small size, and characteristic color
-
promoter:beta-glucuronidase analysis
wild-type and mutant enzymes expressed in Escherichia coli BL21
-