6.3.1.8: glutathionylspermidine synthase

This is an abbreviated version!
For detailed information about glutathionylspermidine synthase, go to the full flat file.

Word Map on EC 6.3.1.8

6.3.1.8

trypanothione

trypanosomatids

gsh

trypanosoma

leishmania

n1,n8-bisglutathionylspermidine

cruzi

polyamine

brucei

gamma-glu-cys-gly

ligases

protozoa

tetrahedral

mgatp

obeys

slow-binding

kinetoplastida

anti-parasitic

phosphinate

atp-grasp

acyl-enzyme

polyamine-containing

bollinger

histidine-dependent

glutathione-like

aminopropylates

mg2+-atp

glutathionylation

drug development

pharmacology

Reaction

Synonyms

bifunctional glutathionylspermidine synthetase/amidase, Gen Bank AE000381-derived protein GI 1789361, GenBank U66520-derived protein GI 1813514, Glutathione:spermidine ligase (ADP-forming), Glutathione:spermidine ligase [ADP-forming], glutathionylspermidine synthetase, Glutathionylspermidine synthetase (Crithidia fasciculata strain HS6 gene Cf-GSS), Glutathionylspermidine synthetase (Crithidia fasciculata), Glutathionylspermidine synthetase/amidase, Gsp synthetase, GspS, GspSA, LiGSPS, Protein (Escherichia coli strain K12-MG1655 gene gsp), Synthetase, glutathionylspermidine, Synthetase, glutathionylspermidine (Crithidia fasciculata fragment), Synthetase, glutathionylspermidine (Crithidia fasciculata strain HS6 gene Cf-GSS), Synthetase, glutathionylspermidine (Crithidia fasciculata), Synthetase, glutathionylspermidine (Escherichia coli clone pJBM1 gene gsp), TryS

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

6.3.1.8 glutathionylspermidine synthase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
578	AA Sequence
4	Application
5	CAS Registry Number
12	Cloned(Commentary)
7	Cofactor
2	Crystallization (Commentary)
4	General Information
1	IC50 Value
37	Inhibitors
8	Ki Value [mM]
41	KM Value [mM]
1	Localization
7	Metals/Ions
9	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
25	Organism
5	Pathway
15	PDB ID
5	pH Optimum
1	pH Range
1	pI Value
15	Protein Variants
10	Purification (Commentary)
5	Reaction
1	Reaction Type
23	Reference
1	Renatured (Commentary)
2	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
41	Substrates and Products (Substrate)
5	Subunits
31	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
10	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 6.3.1.8 - glutathionylspermidine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Substrate/Product Search

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Crithidia fasciculata

P90518, Q5DM89

trypanothione synthase overall reaction

662446

gamma-Glu-Gly-Gly + spermidine + ATP

N1-(gamma-Glu-Gly-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 14% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-Glu-L-Cys(S-butyl)-Gly + spermidine + ATP

N1-(gamma-Glu-L-Cys(S-butyl)-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 24% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-Glu-L-Cys(S-ethyl)-Gly + spermidine + ATP

N1-(gamma-Glu-L-Cys(S-ethyl)-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 77% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-Glu-L-Cys(S-propyl)-Gly + spermidine + ATP

N1-(gamma-Glu-L-Cys(S-propyl)-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 56% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-Glu-L-Ser-Gly + spermidine + ATP

N1-(gamma-Glu-L-Ser-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 16% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-L-Glu-L-Ala-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-Ala-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 28% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-L-Glu-L-alpha-aminobutyryl-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-alpha-aminobutyryl-Gly)-spermidine

Crithidia fasciculata

gamma-L-Glu-L-alpha-aminobutyryl-Gly i.e. ophthalmic acid

966

gamma-L-Glu-L-Cys(S-methyl)-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-Cys(S-methyl)-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 74% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-L-Glu-L-Cys-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate

7 entries

gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2-

N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate

3 entries

gamma-L-Glu-L-Ile-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-Ile-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 70% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-L-Glu-L-Val-Gly + spermidine + ATP

N1-(gamma-L-Glu-L-Val-Gly)-spermidine + ADP + phosphate

Crithidia fasciculata

at 88% of the activity with gamma-L-Glu-L-Cys-Gly

966

gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP

3 entries

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

9 entries

glutathionylspermidine + H2O

glutathione + spermidine

Crithidia fasciculata

P90518

amidase activity

649733

additional information

8 entries