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6.3.1.8: glutathionylspermidine synthase

This is an abbreviated version!
For detailed information about glutathionylspermidine synthase, go to the full flat file.

Word Map on EC 6.3.1.8

Reaction

glutathione
+
spermidine
 +
ATP
=
glutathionylspermidine
 +
ADP
 +
phosphate

Synonyms

bifunctional glutathionylspermidine synthetase/amidase, Gen Bank AE000381-derived protein GI 1789361, GenBank U66520-derived protein GI 1813514, Glutathione:spermidine ligase (ADP-forming), Glutathione:spermidine ligase [ADP-forming], glutathionylspermidine synthetase, Glutathionylspermidine synthetase (Crithidia fasciculata strain HS6 gene Cf-GSS), Glutathionylspermidine synthetase (Crithidia fasciculata), Glutathionylspermidine synthetase/amidase, Gsp synthetase, GspS, GspSA, LiGSPS, Protein (Escherichia coli strain K12-MG1655 gene gsp), Synthetase, glutathionylspermidine, Synthetase, glutathionylspermidine (Crithidia fasciculata fragment), Synthetase, glutathionylspermidine (Crithidia fasciculata strain HS6 gene Cf-GSS), Synthetase, glutathionylspermidine (Crithidia fasciculata), Synthetase, glutathionylspermidine (Escherichia coli clone pJBM1 gene gsp), TryS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.8 glutathionylspermidine synthase

Cloned

Cloned on EC 6.3.1.8 - glutathionylspermidine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination, cloning and RNAi transfection, expression in Escherichia coli
-
enzyme expression analysis, cloning and transfection of RNAi
-
expressed in Escherichia coli BL21 (DE3) cells
expression in Escherichia coli and Saccharomyces cerevisiae
expression of a recombinant histidine-tagged enzyme in Escherichia coli
expression of enzyme mutant GspSA C59A in Escherichia coli strain BL21(DE3)
gene GSPS, a pseudogene with 2 frame shift mutations and 2 stop codons, inactive enzyme, phylogenetic analysis
AJ748279
gene gspS, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gss, DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, transcriptome analyses
overexpression in Escherichia coli and Saccharomyces cerevisiae of the gene Cf-GSS yields insoluble proteins
recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) in inclusion bodies