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6.3.1.19: prokaryotic ubiquitin-like protein ligase

This is an abbreviated version!
For detailed information about prokaryotic ubiquitin-like protein ligase, go to the full flat file.

Word Map on EC 6.3.1.19

Reaction

ATP
+
[prokaryotic ubiquitin-like protein]-L-glutamate
+
[protein]-L-lysine
=
ADP
+
phosphate
+
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine

Synonyms

PafA, proteasome accessory factor A, Pup ligase, Pup-protein ligase, Rv2097c, ubiquitin-like protein ligase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.19 prokaryotic ubiquitin-like protein ligase

Purification

Purification on EC 6.3.1.19 - prokaryotic ubiquitin-like protein ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme expressed in Escherichia coli
recombinant His6-tagged enzyme expressed in Escherichia coli
-
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chormatography
recombinant His6-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chormatography
recombinant N-terminal His6-tagged wild-type and mutant enzymes from Mycobacterium smegmatis or Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration