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6.3.1.19: prokaryotic ubiquitin-like protein ligase

This is an abbreviated version!
For detailed information about prokaryotic ubiquitin-like protein ligase, go to the full flat file.

Word Map on EC 6.3.1.19

Reaction

ATP
+
[prokaryotic ubiquitin-like protein]-L-glutamate
 +
[protein]-L-lysine
=
ADP
 +
phosphate
 +
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine

Synonyms

PafA, proteasome accessory factor A, Pup ligase, Pup-protein ligase, Rv2097c, ubiquitin-like protein ligase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.19 prokaryotic ubiquitin-like protein ligase

Cloned

Cloned on EC 6.3.1.19 - prokaryotic ubiquitin-like protein ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pafA, genetic arrangement in the pupylation gene locus, comparisons, overview
gene pafA, recombinant expression of N-terminal His6-tagged enzyme in Mycobacterium smegmatis, and recombinant expression of N-terminal His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), recombinant expression of HA- or Strep-tagged enzyme mutants in Mycobacterium smegmatis
recombinant expression of His6-tagged enzyme in Escherichia coli
recombinant expression of His6-tagged enzyme in Escherichia coli strain ER2566
reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli
the pafA gene is cloned with a C-terminal His6-tag, expression in Escherichia coli