6.3.1.13: L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

This is an abbreviated version!
For detailed information about L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, go to the full flat file.

Reaction

Synonyms

Cys:GlcN-Ins ligase, cysS2, cysteine ligase, L-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, More, MSH ligase, MshC, MshC ligase, MTCY261.29c, mycothiol cysteine ligase, mycothiol ligase, Rv2130c

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

                6.3.1.13 L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
4285	AA Sequence
8	Application
1	CAS Registry Number
6	Cloned(Commentary)
6	Cofactor
1	Crystallization (Commentary)
2	Expression
11	General Information
2	IC50 Value
26	Inhibitors
1	Ki Value [mM]
6	KM Value [mM]
4	Metals/Ions
3	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
25	Organism
1	Pathway
3	pH Optimum
6	Protein Variants
4	Purification (Commentary)
4	Reaction
17	Reference
6	Specific Activity [micromol/min/mg]
22	Substrates and Products (Substrate)
1	Subunits
38	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 6.3.1.13 - L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

20 entries

additional information

?

-

Mycolicibacterium smegmatis

-

penultimate enzyme in the mycothiol biosynthetic pathway

690918

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis

-

-

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis

-

-

671300

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis

P9WJM9

-

744634

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis

-

enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis

-

the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis

671300

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis ATCC 25618 / H37Rv

P9WJM9

-

744634

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis Rv2130c

-

-

671300

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycobacterium tuberculosis Rv2130c

-

the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis

671300

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

-

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

-

672220, 676970

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

A0QZY0

-

745132

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

a key step in mycothiol, i.e. 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-R-D-glucopyranoside or MSH or AcCys-GlcN-Ins, biosynthesis, mycothiol is produced to act against oxidative and antibiotic stress and is essential for cell growth, biosynthetic pathway, overview

672220

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

key enzyme in the biosynthesis of mycothiol, pathway overview

676970

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

the product 1D-myo-inositol 2-(L-cysteinyl)amido-2-deoxy-alpha-D-glucopyranoside is an intermediate in the biosynthetic pathway of mycothiol. Mycothiol is produced by Mycobacterium tuberculosis to maintain an intracellular reducing environment and protect against oxidative and antibiotic induced stress. The biosynthesis of mycothiol is essential for cell growth

690956

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis

-

positional isotope exchange analysis confirms that MshC catalyzes the formation of a kinetically competent cysteinyl-adenylate intermediate after the addition of ATP and cysteine

690956

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis mc(2)155

-

-

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis mc(2)155

-

enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress

650166

-

650166

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Mycolicibacterium smegmatis mc2155

A0QZY0

-

745132

-

-

?

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP

1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

Rhodococcus jostii

-

inactivation of the mshC gene in RHA1 results in the accumulation of the MSH pathway intermediate, 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside. The mutant is deficient in the biochemical degradation of a number of xenobiotics metabolized by the parent strain, it shows increased susceptibility to a number of antibiotics and it shows unusual growth characteristics, exhibiting a long lag phase before normal exponential growth

695095

-

-

?