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6.3.1.13: L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

This is an abbreviated version!
For detailed information about L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, go to the full flat file.

Word Map on EC 6.3.1.13

Reaction

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
+
L-cysteine
+
ATP
=
1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol
+
AMP
+
diphosphate

Synonyms

Cys:GlcN-Ins ligase, cysS2, cysteine ligase, L-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, More, MSH ligase, MshC, MshC ligase, MTCY261.29c, mycothiol cysteine ligase, mycothiol ligase, Rv2130c

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.13 L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Purification

Purification on EC 6.3.1.13 - L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2400fold, 2 peaks
-
recombinant enzyme from mshC-deficient mutant strain I64 of Mycobacterium smegmatis by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration
-
recombinant GST-tagged MshC and recombinant maltose binding protein-MshC by affinity chromatography on glutathione and amylose resins, respectively
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration to about 95% purity
-