6.2.1.B3: OPC-8:CoA ligase
This is an abbreviated version!
For detailed information about OPC-8:CoA ligase, go to the full flat file.
Reaction
Synonyms
4-coumarate--CoA ligase-like 5, 4-coumarate-CoA ligase-like 5, OPC-8:0 CoA ligase 1, OPC-8:0 CoA ligase1, OPCL1
ECTree
6.2.1.B3 OPC-8:CoA ligaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 6.2.1.B3 - OPC-8:CoA ligase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
12-oxo-phytodienoate + CoA + ATP
12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
-
-
?
12-oxo-phytodienoic acid + CoA + ATP
12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
decanoic acid + CoA + ATP
decanoyl-CoA + AMP + diphosphate
-
-
-
?
dinor-12-oxo-phytodienoic acid + CoA + ATP
dinor-12-oxo-phytodienoyl-CoA + AMP + diphosphate
-
-
-
?
dodecanoic acid + CoA + ATP
dodecanoyl-CoA + AMP + diphosphate
-
-
-
?
hexadecanoic acid + CoA + ATP
hexadecanoyl-CoA + AMP + diphosphate
-
-
-
?
octadecanoic acid + CoA + ATP
octadecanoyl-CoA + AMP + diphosphate
-
-
-
?
tetradecanoic acid + CoA + ATP
tetradecanoyl-CoA + AMP + diphosphate
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
i.e. OPC-6:0
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
i.e. OPC-8:0
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
i.e. OPC-8:0
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA is subsequently converted to jasmonic acid via three beta-oxidation steps in the plant metabolism
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
i.e. OPC-8:0. In leaf peroxisomes, 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate is a physiological substrate for the activation of jasmonic acid biosynthetic precursors
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
i.e. OPC-8:0
-
-
?
additional information
?
-
enzyme is involved in conversion of the C18 jasmonic acid precursor 12-oxo-phytodienoic acid to 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid, i.e. OPC-8:0, which subsequently undergoes three rounds of beta-oxidation to yield jasmonic acid. Enzyme readily accepts 14:0 and 18:1 fatty acid substrates but has little or no activity with shorter chain substrates such as hexanoic and octanoic acids
-
-
?
additional information
?
-
recombinant OPCL1 is active against both 12-oxo-phytodienoic acid and 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid, as well as medium-to-long straight-chain fatty acids. OPCL1 readily accepts 14:0 and 18:1 fatty acid substrates, cf. EC 6.2.1.3, but has little or no activity with shorter chain substrates such as hexanoic and octanoic acids
-
-
?
